FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21356242
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC410260.1921030863014195No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC397080.18593158852573405No Hit
GTAGTAACAAGAGCATAGGTCCTGAGGTAGCAGCTCCTGGTGGGGTTCAG291730.13660174856606325No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC278650.13047707550794752No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG276500.12947034408019914No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA259180.12136030299712842No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT255590.11967929563637648No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA231780.10853033038303274No Hit
TCTCTACACTGATGTCTAATGAGCTCCTCAAATTTATGTCCAGAATGGAG227390.10647472528172326No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT223250.104536181974338No Hit
ACAACAGCAGGGCATGTATGAGTAGTTGAGAACGGTGAATAGGAGTATGA215680.10099155085431229No Hit
AGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACAACGCCCGGGTTA215260.10079488704051959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA607100.078.3055041
TAGTAAC615850.077.112542
CACGCAC83900.066.022213
CGTGCAT23450.065.856013
TCCGCGA1051.6370905E-1162.1458174
AGCACGC93850.060.2662161
GTAACAA808300.058.088874
TAACAAG817300.057.3516245
GCGGTGC51650.054.3318181
ACGTGCA75300.053.9202272
CGCGAGG1353.6379788E-1253.7062576
AAGAGCG203900.053.396879
AGTAACA910650.052.722543
ACAAGAG910000.051.4501887
TCCGTGC22600.049.073398
CGGTGCG10900.048.5573582
CAAGAGC903000.047.8513768
CCAGCGG159700.047.296168
AACAAGA994800.047.1330766
CAGCGGA161050.046.989729
Produced by FastQC (version 0.11.5)