FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444657
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC73540.2134900514042472No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC65040.18881415479102853No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT57980.16831864536875515No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT55770.16190291224931827No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT55430.16091587638478955No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT55000.15966756632082674No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG54730.15888374372252448No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA52140.15136485287214374No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT47100.13673349770383522No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA46080.133772390110249No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA45770.1328724456455316No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT45220.13127576998232335No Hit
GTAGTAACAAGAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA44820.13011455131817187No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA44500.12918557638685071No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA42560.12355366586571609No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA42420.12314723933326308No Hit
AAAATGAACAATGCTACCTTCAACTATACAAACGTTAACCCTATTTCTCA40870.11864751700967616No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA40750.11829915141043071No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT40580.11780563347816633No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG39770.11545416568325961No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA37980.1102577121611818No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC37520.10892231069740761No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA36460.10584508123740621No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC36000.10450967977363203No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT35440.10288397364381999No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA35260.10236142524495183No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA35110.10192596824589503No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC34840.10114214564759277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC100.0070960023144.96997145
CGGTGCA4900.0110.965742
GGTGCAT5350.090.7914053
TACGGAT300.001936398872.497613
GCACGCA10350.067.244162
CACGCAC10850.064.813543
ACGTGCA14800.064.6600342
GTAACAA136800.063.54144
CATACGG350.003565657262.1498341
TAACAAG141150.061.583165
AGCACGC11900.061.540521
GGTGCGT7500.059.9313623
CGGTGCG8600.056.4806942
TAGTAAC161350.054.4125252
GTGCACG400.006052925354.3732074
CGCCAAC400.00605709854.36374145
AGTAACA168000.052.3450053
CAGCGGA604.952188E-448.322629
CGGAGCA13050.046.1095962
CCAGCGG657.347108E-444.6054958
Produced by FastQC (version 0.11.5)