FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951776
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC109600.37130188740609044No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT96300.3262442678577236No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC70480.23877150569690925No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA66970.22688035948527258No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT62820.21282102706980474No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT61690.20899282330366534No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG50010.1694234250837462No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA45260.15333141810218662No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC44880.15204405754366185No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG42630.14442152792081786No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG41460.14045781251693895No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA40180.1361214401092766No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG36370.12321395661459406No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGATCCCGGGCAAGATAAACGAGGGT36070.1221976193315482No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC34950.11840329347484362No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA33970.1150832583502271No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC30090.10193862948950055No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG29670.10051575729323635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC17350.0124.122551
GCACGCA17500.0123.4625242
CACGCAC18850.0115.005013
CGGTGCA8750.0106.061762
GCGGTGC13900.098.591791
TAACAAG86250.093.224355
GTAACAA86800.092.6336444
CGCACTT23600.092.4722445
AAACACG21250.092.121665
ACGCACT24650.090.298054
GAAACAC22300.088.109224
ACGTGCA10750.084.3062
CGTGCAC950.083.951033
AACACGT6600.079.094366
TAGTAAC103250.078.226172
GTAGTAA109100.075.300681
GAAGCGG6750.075.194831
AGTAACA109200.074.096633
ACAACGC6500.072.485978
CCACTAT10100.065.3091358
Produced by FastQC (version 0.11.5)