FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639752
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT132850.8101834911620781No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT92310.5629509828315502No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT84990.5183100859154312No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT79690.48598812503354166No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT69850.425979050490562No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA66770.40719572227995454No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC65850.40158511774951333No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT65510.3995116334665242No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT50140.3057779469090448No Hit
AGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA49620.30260673565270846No Hit
CACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA45580.2779688635842493No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT41040.2502817499231591No Hit
GCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAAC38520.23491357229629847No Hit
GAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC38440.2344256936414775No Hit
GCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAAC37780.23040069473920446No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34440.21003176090042883No Hit
CATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTGAAAAGGAAAAGGT31780.1938097956276315No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT31690.19326093214095788No Hit
GTATAGTGCTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAA31190.19021169054832682No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA30230.18435714669047515No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT29600.18051510228376No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG28810.17569730056740288No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT28610.17447760393035044No Hit
AAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACA28310.17264805897477178No Hit
GGATGAAGTGGGCCATGTGTTCTAACTTTCCCTTGGCTCTACCCAGATCT26530.16179275890500514No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25670.1565480633656797No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC25200.15368177626860646No Hit
ACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAA24770.15105942849894374No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC24570.1498397318618913No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC24540.14965677736633345No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT24260.14794920207446005No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG23670.14435109699515536No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT23530.14349730934921867No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT23140.14111890090696644No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT23140.14111890090696644No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT22980.14014314359732447No Hit
CAACAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA22210.1354473115446726No Hit
GCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGTG22180.13526435704911474No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC22100.13477647839429377No Hit
AACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATT21680.13221511545648365No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT21410.13056852499646288No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA21260.12965375251867356No Hit
GTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT21220.12940981319126305No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT20340.12404314798823238No Hit
GCTCTACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGC20310.12386019349267449No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT19720.12026208841336983No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA19720.12026208841336983No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA19620.11965224009484361No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT19250.11739580131629661No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT18700.11404163556440242No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC18490.11276095409549737No Hit
AGCACGCACTTGTGAATCTGATGGGGACAAAGGGAGTGGAGGCATCAAGG18050.11007762149398202No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG17750.10824807653840338No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC16990.10361322931760414No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC16990.10361322931760414No Hit
GACCAATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAA16950.10336928999019362No Hit
GCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAACAACAGTAGACC16680.10172269953017286No Hit
CACGTGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAATGA16660.1016007298664676No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG100.007092557144.991235
TAGCGTT100.007092557144.991233
GTAGCGT100.007092557144.991232
GTAACAA48450.0129.130464
AGCACGC12500.0126.4400561
TAACAAG51000.0122.531795
GCACGCA13150.0119.631542
CGGTGCA3000.0118.409492
CACGCAC13300.0118.282323
GAAACAC21450.0112.207664
GAGCAGA4450.0109.157351
GACAGGG203.8664963E-4108.7500461
GTCGTCC203.8674346E-4108.7434163
AAACACG22850.0105.332795
TAGTAAC61550.0102.11812
TAGAAAC25600.098.265532
AGTAACA64650.097.670033
ACGCACT16300.097.402074
GTAGAAA25800.097.228731
CGCACTT16400.096.808175
Produced by FastQC (version 0.11.5)