FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487770
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA122340.3507685426504615No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA70070.2009020090200902No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT57890.16597998147813645No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG50060.1435301066297376No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG47590.13644821762902945No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC39060.11199132970350684No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC38710.11098782316494493No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA36930.10588427562597304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA208050.056.0836871
TAGTAAC208750.055.9199332
GTCGTCC11300.050.689253
GTAACAA231700.050.0994034
AGTAACA240600.048.4872633
AAGAGCG18850.048.4543849
CTGTCGT11700.048.34561
TAACAAG243300.047.7703745
TGTCGTC12050.047.5343172
ACAAGAG251700.044.467017
CAAGAGC229350.044.1857538
CGTCCAG13200.043.393075
AACAAGA260350.042.9710166
GCTATCG855.3488984E-542.640767
CGTGCAC15800.042.2180373
CAGGCGA15900.039.2080429
GCACGCA35050.038.062482
CGGTGCA9800.036.9922872
CACGCAC37600.036.0596123
CCACTAT20100.035.703688
Produced by FastQC (version 0.11.5)