FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476451
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG322610.9279866162359257No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA157550.4531920628249902No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA156100.44902114253875575No Hit
GTAGTAACAAGAGCCTCTGGTTGAGGTCCTTAACTTCAGAATTTCCCTCT139810.40216301049547365No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG129080.37129820037733885No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG128120.36853676349817677No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT112730.32426747852910914No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA99540.2863264864081214No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT86610.2491333834419067No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA79290.22807742723829563No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT77780.22373391714711355No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG75010.21576602115203122No Hit
TTCTAATGCATAGCCAAAGTCAAGAATCACTAGAGCTCTCTTTAAAATTC72410.20828712960430049No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA70800.2036559698382057No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG68250.19632090312793135No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT66520.19134456375194128No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT65960.18973372557243004No Hit
AACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC65630.18878448164521808No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG64150.1845272664565098No Hit
CTTCATAATCAGAACAAACATGTGATTGAGTGGAACAAGCTAGTGCTAGC63900.183808142269228No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG60260.17333769410240502No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC59160.1701735476783651No Hit
AGTTCACACACTGCCTGAACATTATAATCGCCTAGGGAACAACACACACA57170.16444931914760197No Hit
AAACAACAGCGATCCGAAATCCCCACTGCATAGACAGAAAGGCTGAGGCC56700.1630973656755122No Hit
CACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATGCA54060.15550341425781639No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC53560.15406516588325278No Hit
CTCCTTGACACCTGACACAATACCTTCCCACAATAGCCACTTCATAATCA52620.1513612589390732No Hit
ACACAATACCTTCCCACAATAGCCACTTCATAATCAGAACAAACATGTGA52620.1513612589390732No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC52470.15092978442670413No Hit
CACACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATG52290.15041201501186122No Hit
CACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATGCATA51750.14885870676733254No Hit
GTAGTAACAAGAGCAGGGGGAGGAGGTAACGATTGGTTCACTGCTTAATT51190.14724786858782132No Hit
TTACTATGCCTCATTGAGCCATGGTTTGCCCATCTTGAGGTGGACATGCC49780.14319200817155198No Hit
CACACACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAA49570.14258794385423526No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA49400.14209893940688362No Hit
GTTCACACACTGCCTGAACATTATAATCGCCTAGGGAACAACACACACAC49330.14189758463444474No Hit
GAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCC48800.1403730413574073No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG46040.13243391032981625No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC46000.13231885045985115No Hit
GAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGCATGAG44390.12768769069375635No Hit
CTTTAAAATTCATATGTGACATATCCTTTTAACCCCAAAACAACCACACT44320.12748633592131747No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG42680.12276888125274885No Hit
GAACAAGCTAGTGCTAGCCCTCCCTTTTCCACTAATTTGATGCATGATAT42650.12268258635027504No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA42530.12233740674037978No Hit
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC42330.12176210739055432No Hit
CACACACACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCT42130.1211868080407289No Hit
TCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTGTA41850.12038138895097328No Hit
TTCCTTATCTTTTCATAGCCCCTAGTTCACACACTGCCTGAACATTATAA41620.11971979469867403No Hit
TTTTAAAGCTTTGTAGGTGATTCTAATGCATAGCCAAAGTCAAGAATCAC40940.11776377690926752No Hit
AGCTAGTGCTAGCCCTCCCTTTTCCACTAATTTGATGCATGATATTTGGG40880.11759118710431989No Hit
AACACGAGCCTCTGGTTGAGGTCCTTAACTTCAGAATTTCCCTCTAGATC40810.117389832331881No Hit
ATATATTCCTATAAATTTAATTTTCTTGCTGACTTCATCCTTGGAGTTCT40400.11621046866473884No Hit
CCTTGACACCTGACACAATACCTTCCCACAATAGCCACTTCATAATCAGA40290.11589405402233485No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC39370.11324767701313783No Hit
CACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATGCATAGC39210.11278743753327747No Hit
CTTAAAATCTACTCTTTTAGCAAAAATCCTGAATACAAATAATTTTATTA38750.11146424902867896No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG38580.11097524458132733No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT37870.10893293188944703No Hit
GGAATATATAGTGTCTTTGAACACACAAAGTGGGGAATTATATTTCTAGC37640.10827133763714777No Hit
ATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCTAG37640.10827133763714777No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT36440.10481954153819513No Hit
GTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTA36210.10415794728589588No Hit
ATTCTAATGCATAGCCAAAGTCAAGAATCACTAGAGCTCTCTTTAAAATT35100.10096503589436469No Hit
ACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCC35080.10090750595938214No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAT100.007091479145.000922
GTAGTAA152100.097.2546541
TAGTAAC153300.096.4780962
CGAGCCG4050.087.716595
TCGCGTG259.3949237E-486.98553145
GTAACAA176650.083.027694
TAACAAG178850.081.398335
CGGTGCA2550.079.608342
CGAGCTA5750.076.913525
AAGAGCG24950.073.213229
AGTAACA206150.071.638823
CGTGCCG2300.069.348265
AACAAGA211400.069.036616
AAGAGCC113550.068.624769
CAAGAGC206900.067.827278
GCGGTGC9500.066.405661
ACAAGAG222750.065.506687
GAAGCGG8750.063.8104931
CGCACTA702.3361281E-762.1432462
AGCACGC5750.061.7927741
Produced by FastQC (version 0.11.5)