FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261220
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA94260.41685461830339376No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT89090.39399085449447646No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC76870.33994923094612645No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT67900.3002803796180823No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA58380.2581792129912171No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG37410.16544166423435136No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC35630.15756980744907617No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA34750.15367810297096257No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT29990.13262751965753No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA29210.1291780543246566No Hit
CACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG28810.12740909774369588No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT28290.12510945418844696No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG27950.12360584109463034No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG27600.1220580040862897No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG25550.112992101608866No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA25540.11294787769434199No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG23850.10547403613978294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC26050.0120.521541
GCACGCA27300.0114.9853442
CACGCAC30250.0103.7719043
GTAACAA84700.0102.624974
CGGTGCA6000.0100.286882
TAACAAG88400.098.2475745
CGCACTT33100.098.122215
TAGTAAC90150.096.6620562
GTAGTAA91650.096.2814561
ACGCACT34150.095.954434
GAAACAC26050.093.786314
AAACACG26350.092.718545
AGTAACA97350.089.736343
GTTCGAT608.3127816E-1084.57932
GCGGTGC11800.082.95381
ACGTGCA18950.082.252012
CTCGCTC9350.079.0871353
GTCTCGC9500.078.613681
GGTTCGA651.5643309E-978.085281
CGAGCTA5100.073.918045
Produced by FastQC (version 0.11.5)