FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741515
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT209200.7630817267095019No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC135510.49428874180881743No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT104550.38135848244492554No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT85170.3106676417965979No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC80690.2943263122762414No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA72740.26532774761400174No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA66740.24344203843495293No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG53530.19525700205908048No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT52020.1897490985823532No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT50630.18467890928920688No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT46440.1693953890458378No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA43870.1600210102808119No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT41080.1498441555125542No Hit
CACGTGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA40470.14761910841268422No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG39460.14393501403421102No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC38530.14054272911145843No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA37480.1367127300051249No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG35230.1285055890629816No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA34110.1244202566828925No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA32300.11781806774721276No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA32290.11778159156524769No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG32280.1177451153832826No Hit
AGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAACAA31960.1165778775604No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG31870.11624959192271427No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT31360.11438930664249512No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG31340.11431635427856496No Hit
GCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGTA31310.11420692573266972No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC31140.11358683063926332No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31060.11329502118354268No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC30500.11125235499349813No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTATCCAAT30270.11041340280830125No Hit
GCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAACAAC29830.10880845080183767No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA29540.10775064152485031No Hit
CACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA29230.1066198798839328No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT29190.10647397515607246No Hit
ACAACAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAA28230.10297226168742467No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC100.007088225145.022611
AGCCGTT100.0070897713145.012043
GCCGTTA100.0070897713145.012044
AGCACGC34150.0129.52241
GCACGCA34950.0126.755882
CACGCAC35450.0124.968073
CGGTGCA5300.0124.491472
GAAACAC19150.0116.23684
CGCACTT42450.0107.776915
ACGCACT42950.0107.028684
AAACACG20950.0105.903785
GTAACAA82850.0105.892924
TAACAAG90250.097.611985
ACGTGCA16550.091.12542
GTAGTAA103700.085.7269741
TAGTAAC103200.085.222672
AGTAACA105750.083.1676563
CAGCGTC451.2328528E-680.562242
ACAGCGT900.080.562244
GCGGTGC18050.078.7380451
Produced by FastQC (version 0.11.5)