FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641396
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC138640.5248739681592612No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC71400.2703116079527644No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT67320.25486523035546355No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT48940.1852808136303682No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA45510.17229525599342166No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT44440.16824436775099227No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG42910.1624519761520045No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC40680.1540094707495582No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA37900.14348473307296594No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT36280.1373516125563906No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA36130.1367837310270781No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA35830.13564796796845305No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT32860.12440391368806496No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA29780.11274341295284765No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG29700.112440542803881No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA29260.11077475698456424No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT28640.10842751333007244No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT28400.10751890288317237No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT28310.10717817396558486No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA27920.10570168198937228No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG27230.10308942695453464No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA27130.10271083926832629No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT27090.10255940419384296No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA27040.10237011035073877No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26830.10157507620970124No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC26430.10006072546486781No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAA100.007091342145.001196
GTACGGC100.007091342145.001194
GCGACCG202.1327814E-6144.97923145
CGTGCAC403.274181E-11126.8760453
GTAACAA58250.0117.245594
AGCACGC4950.0108.405251
CGGTGCA6000.0107.542552
GGTGCAT5550.0105.811683
TAACAAG67250.0101.878155
CGGAGAT607.2759576E-1296.685771
GCACGCA5500.096.2280652
GCGTCAG1150.094.58391
CTCGCTC1150.094.5659943
CACGCAC6000.088.209053
GAAACAC20700.086.860614
CGTCAGA1300.083.654532
GCGAAGC353.4381836E-582.857823
AAACACG21950.081.9141165
ACGTGCA10250.079.927492
GTCTCGC1400.077.693921
Produced by FastQC (version 0.11.5)