FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760765
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC20970.2756435955912798No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20240.26604799116678607No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT19710.25908131946133167No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG16110.21176053051862267No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC15250.20045611982675332No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA13480.17719006526325476No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT12380.16273093530853813No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA12000.15773596314236327No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA9860.12960638304864183No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC9610.1263202171498426No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT9610.1263202171498426No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG9540.12540009069817878No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG9420.12382273106675518No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG8040.10568309530538339No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG8000.10515730876157553No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA7890.10371139576610384No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC7840.103054162586344No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG100.00709005145.00331
GGTCACG550.0131.821171
CGGTGCA2300.0104.017262
GTCACGA700.0103.566972
GTAACAA22800.0102.067974
CGGGGGG301.5995538E-596.6625064
CATCCGA900.096.66252
CTGTCGC451.0926669E-896.6497969
AGCACGC3700.096.0156861
ACGTGCA1600.095.152162
TAACAAG25200.092.0595255
ACGAGAT800.090.62115
GCACGCA4100.086.642612
TAGTAAC27550.085.259482
GTCTCGC608.2945917E-1084.585251
CACGCAC4300.084.29873
GAAACAC5100.083.8689354
AAACACG5150.083.054675
GTAGTAA29350.082.0120851
CCGAAAC1100.079.08755
Produced by FastQC (version 0.11.5)