FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4202061
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT77140.18357658301485866No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA71260.169583449645305No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT66340.15787490947894378No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA64110.1525679898506947No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT56080.13345831961982466No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT52730.12548604125451773No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT47100.11208785403162877No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG44370.10559104211005027No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCGA400.00605571554.367085145
AGCATCG604.950452E-448.3263145
TAAGGGT1105.2671403E-639.5467574
GTAGCGC1301.65472E-533.465431
CTACAGA5100.032.697164
GTCTAAG1153.1616527E-431.5254061
CAACGCA86500.029.4147959
ACGTGCA16800.028.0515212
CGCTGAG1606.831724E-527.1883965
TAGATGT4600.026.7943636
AACAACG101650.025.815237
TAGCGCT1701.0318312E-425.589082
ACAACGC103050.025.4645148
AGCGTTT2901.19252945E-825.0008246
GCGCTGA1751.2563798E-424.8579644
CGGTGCA13450.024.7963562
TGCGATA2701.653807E-724.16315145
TGGGCCG18100.024.0339435
TCTAAGG1550.001809101923.3878692
GTAACAA350600.023.0576654
Produced by FastQC (version 0.11.5)