FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072616
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA34710.3236013633956607No Hit
AGCACAGCCACTATGCTTGATTAGCATTACAGAGATGGTACAATGATGGT26920.25097518590063916No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA24820.23139688388015842No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG23880.22263326297575275No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT22140.2064112413016401No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT20600.1920538198199542No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20560.19168089978146885No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA20450.19065536967563415No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC20190.1882313894254794No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG19660.1832901989155485No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19230.17928130850183105No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA19060.1776963983382683No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC16940.15793163629854487No Hit
TTGTCCAGCCGTCCCTACCATCCATCTCCATAATTCGTTTCATCTTGTAA16500.15382951587520605No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT15000.13984501443200548No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACGATGATGGT14620.13630227406639467No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14550.13564966399904532No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC14470.13490382392207462No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC14330.13359860378737592No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT14030.1308017034987358No Hit
GTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTGCTCCT13500.12586051298880493No Hit
GTATTTTTTAATGGGTATAGAGTTTCAGTTTTACAAGATGAAACGAATTA13190.12297038269054349No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA13030.1214787025366021No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG12560.11709689208439927No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG12310.11476614184386584No Hit
CACTTGGTCCATGGGGCTCCGCTTCCTTCCCATGGCCTCCCCTCTGGGGT11980.11168955152636173No Hit
GTACAATGATGGTTGCCAGAAGCTGGGGGGAGGAAGAAATGGGGAAGTAT11890.11085048143976968No Hit
ACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGAT11000.10255301058347069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA35400.0107.519214
AGCACGC6850.0101.608711
GCACGCA7200.096.6648942
TAGTAAC40650.093.989662
AGTAACA44350.086.3118443
TAACAAG44600.085.8280265
GAAACAC12200.084.38374
GTCGCCC1450.079.987349
CGGTGCA5000.078.298572
CAAGAGC41550.077.809598
CGCACTT8150.077.391225
CACGCAC9100.076.482123
ACAAGAG50100.075.961047
GTAGTAA52150.074.656951
AAGAGCG4750.073.251589
ACGCACT10700.072.498674
CGTCTAG300.001936995772.488537
CGTGCAC1950.070.639733
AACACGT5650.069.290776
ACGTGCA7050.068.8994452
Produced by FastQC (version 0.11.5)