FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904188
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA75850.3983325175875492No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC45620.2395771846057217No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA42170.22145922566469278No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT40490.21263656739775694No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG39100.20533686799832787No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT35180.18475066537547763No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC33850.17776606091415345No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG32530.1708339722758467No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC30420.15975313361915944No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA29740.15618205765397114No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC29220.15345123485706244No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29140.15303110827292263No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC27580.1448386398821965No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC25120.13191974741989762No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT24430.12829615563169183No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA23110.1213640669933851No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT22860.12005117141794823No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG21760.11427443088602596No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC21740.11416939923999102No Hit
AGCACGCACTTTACATACATGTTATTTCCCTTCACAGTAACCCCTGGGAC21250.11159612391213472No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA20820.1093379435223833No Hit
CTCTTGACCATTTTAAGTGTACAGTTCAGTGGTACTAAATAGAGTCATAA20660.1084976903541037No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG20410.10718479477866681No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACAATGATGGT19870.10434894033572316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC100.0070922025144.994253
GCACGCA15850.0109.317432
AGCACGC15800.0108.75141
GTAACAA68300.0104.6590964
CGCACTT16500.0102.3747255
TAACAAG69900.0101.9523245
TAGTAAC71750.099.727752
ACGCACT19050.098.565654
CGGTGCA5950.096.256692
CACGCAC18800.092.5495153
AGTAACA77900.091.85453
AAACACG23950.082.9403465
GAAACAC24200.082.083534
ACAAGAG90500.079.051397
GTAGTAA95650.075.646551
TGCGGCG406.666042E-572.497125
GTCTCGC5350.071.823361
CTCGCTC5650.070.5724263
CAAGAGC83300.070.4824458
TAGAAAC39000.068.965212
Produced by FastQC (version 0.11.5)