FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2774142
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT126380.4555642789734628No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC123670.44579549280462216No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT74680.26920035095535844No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT70690.2548175255628587No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA67750.2442196542210168No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC67570.24357080495518973No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA57510.2073073404317443No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG55350.19952114924181963No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA49290.17767655729230875No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC39540.14253055539334322No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT38790.1398270167857305No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG38310.13809675207685834No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA36890.1329780523131116No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA34870.12569652166327464No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA34320.12371392668435863No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA32510.11718938684465324No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA32040.11549516931721591No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA31570.1138009517897786No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC31340.11297186661677738No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT30860.1112416019079052No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC29800.10742060067581256No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC29800.10742060067581256No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT29440.10612290214415845No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT28700.10345541071798055No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC28650.10327517481080638No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT28370.10226585373063095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC23200.0126.2781141
GCACGCA23900.0122.854212
CACGCAC24800.0118.395793
CGGTGCA4650.0109.138512
GAAACAC18400.0105.596594
TAACAAG78700.098.200885
AAACACG20850.093.536075
ACGTGCA12250.089.36632
CGCACTT37850.087.9183965
ACGCACT38000.087.380564
CATCGCT259.3943323E-486.98644145
GTAACAA90600.085.062484
GTAGTAA99250.078.690061
AACACGT7050.075.070046
GCGGTGC17050.071.8781741
TAGTAAC108950.071.201552
AGTAACA112650.068.862943
CAAGAGG33350.068.031658
GAAGCGG7950.067.4993061
ACAAGAG115400.067.273837
Produced by FastQC (version 0.11.5)