FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT80.2714625042416016No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC70.23752969121140144No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT50.16966406515100102No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC40.1357312521208008No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG40.1357312521208008No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC40.1357312521208008No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA40.1357312521208008No Hit
CCATCATACTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACAT30.10179843909060061No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA30.10179843909060061No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG30.10179843909060061No Hit
GCTAGAATCAGGCCCTTCTTTTTTGTTGCTGTTGTTCCCATTCCTGATAG30.10179843909060061No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30.10179843909060061No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA30.10179843909060061No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT30.10179843909060061No Hit
ATCCTGAGGTTCCAAGTCTGTAGGGTCCTCCTGGTGCTTTTTCTGCATCG30.10179843909060061No Hit
ATAAATGGCTGTTGCTGCTGAGTGCCAAGTCGTTTTCCCACCATCATGTA30.10179843909060061No Hit
CCATATAAACAATAAAAATAGCTAGCATCAATGTTACAGCCAAACTAGAA30.10179843909060061No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT30.10179843909060061No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG30.10179843909060061No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA30.10179843909060061No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC30.10179843909060061No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC30.10179843909060061No Hit
CCTTTTTGGCTGAATTTATCAGTAGTTTTTCCAGTTTCATTTTACTCTTT30.10179843909060061No Hit
TAGAAGTACCATACATTTGTACAGAAGGAGAAGACCAAATTACCGTTTGG30.10179843909060061No Hit
CAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGC30.10179843909060061No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG100.006732901145.02
GAAGAAG100.006732901145.01
AATAGAG100.006732901145.05
TAGAGGG100.006732901145.07
GAGGGGA100.006732901145.09
AAAAAAA2302.1338472E-58.195652125-129
Produced by FastQC (version 0.11.5)