FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918933
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC130720.44783487664841914No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT84610.28986619425659993No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC74210.25423673650611367No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51720.17718803412068723No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA49140.1683491878710474No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA47910.16413531931017258No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT44790.15344648198502672No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT43130.14775947238254528No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG43100.14765669510057272No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA40350.13823544425308837No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA40280.1379956305951524No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA36200.12401792024688474No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA34090.11678925141481494No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC33930.11624110591096129No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA32880.11264390104192183No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT31130.1066485595935227No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA30460.1043532002961356No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC30260.10366801841631856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC9100.0123.494991
GTAACAA54300.0122.831514
CACGCAC9400.0121.085913
GCACGCA9450.0120.445242
GAAACAC17350.0116.1627964
CGGTGCA5850.0115.2521062
AAACACG18650.0106.89955
TAACAAG64850.0102.513525
TAGTAAC77350.086.790552
CGCACTT13150.084.350465
ACGCACT13650.083.916284
TAGAAAC27200.081.825982
ACGTGCA12350.078.074012
AGTAACA87150.077.197353
CGTGCAC502.3078628E-672.49733
CGCACTG502.3078628E-672.49735
AACACGT5600.072.497296
AACACGA21950.072.332156
CAACGCA5250.070.416299
ACAAGAG97400.068.542987
Produced by FastQC (version 0.11.5)