FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11209140
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27050.066.042881
TAGTAAC72150.058.478932
GCAAAAG115550.056.7715233
GAGCAAA121050.055.475981
CGAAAGC42700.053.4507183
AGCAAAA145050.045.858572
CAAAAGC153950.043.2224244
TAACAAG98400.042.7618375
ATATCGT22700.042.6679571
TATCGTG23550.041.1340332
CGCACTT12250.036.3420525
ACGCACT16250.035.3383064
ATCGTGT28800.034.3906943
AAAAGCA217650.030.978535
AAAGCAG272650.030.5376346
TAGCCGT28250.030.0228619
ATAAGTG55700.029.9258868
GTAACAA144850.029.2872144
ATATAAG58050.029.2056736
CACGCAC15400.028.8951723
Produced by FastQC (version 0.11.5)