FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_SMART_NEGCONTROL_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_SMART_NEGCONTROL_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG50.2748763056624519No Hit
CCTTTATACAAAGGAGCAGATATGCAGAATGAATAAGTCTAGAAGATCTA30.16492578339747113No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC30.16492578339747113No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA30.16492578339747113No Hit
GTATGTAATCATCGCCAAAAACATTCGGGGGTTTTGATTTTCATTCCACT30.16492578339747113No Hit
AGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTCAATGCGTCTTGGTTC20.10995052226498077No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT20.10995052226498077No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA20.10995052226498077No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG20.10995052226498077No Hit
TGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATC20.10995052226498077No Hit
CAAATATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAGGGAGTAAG20.10995052226498077No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT20.10995052226498077No Hit
AGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCAC20.10995052226498077No Hit
GGACACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCC20.10995052226498077No Hit
ATTTAATACATTTAGACTAATTTATCACAGTTTGCATTCCATCTTGGGTT20.10995052226498077No Hit
CCCCCAAGATCAAAGGTCCCAGGTTCGAGGTTGTCCCTAAGAGCCTGAAC20.10995052226498077No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT20.10995052226498077No Hit
GTTGAGACCATGGTCATTTTAAGTGCCTCATCAGATTCTTCTTTCAGAAT20.10995052226498077No Hit
GAAAGAATAGGTACACGTTTCCTTCAACATTTTCAAAGAATCAGCTCATG20.10995052226498077No Hit
GATTTAAATGTGTCTAGATATGGATCTCTTTATATTACTTGAAGTTTACT20.10995052226498077No Hit
CCTTATGCAAGCAGAACTTGTTCCGTTTTGAGTGACTCCAGTCCAATTGA20.10995052226498077No Hit
ACCTTGCAGGGTTTCATGTGAAGATGCACACTGAAGTGCTTTGGAAAAGC20.10995052226498077No Hit
GGATTGGGGCTCATTCCCATGTCCCCTCCCTCATGACCTCCCAGAGCCAC20.10995052226498077No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT20.10995052226498077No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC20.10995052226498077No Hit
ATCATGTAGTTGTACTAAATATATTTCTTAATCTGTAATGATAATGTGAT20.10995052226498077No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC20.10995052226498077No Hit
ACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAG20.10995052226498077No Hit
GGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAA20.10995052226498077No Hit
ATTCTCCTGTATTCAACTACAACAAGACCACTAAAAGGCTAACAATTCTC20.10995052226498077No Hit
CGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATG20.10995052226498077No Hit
CTACTGTTCCTAAAGGTTCCAGCGCAAAATGCCATAAGCACCACATTCCC20.10995052226498077No Hit
GAAGAGAGAAGTACTTTAAAGTTTTTGTTTATCCTTTATACAAAGGAGCA20.10995052226498077No Hit
GTACTAAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCC20.10995052226498077No Hit
CAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGG20.10995052226498077No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA20.10995052226498077No Hit
ATTCATCTGTTGACGAGTCTGCAAGTTCTGCTTTCAAAAAATATGGAAAT20.10995052226498077No Hit
GATAGAAACTGCTTTTAAAAATAAGACAAATAGAAATTCTGAAGTTGATG20.10995052226498077No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA20.10995052226498077No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG20.10995052226498077No Hit
GACTGAAGGAGGCAACGTCTCATCTCCATTCCCCATTTCATTTTGACCTT20.10995052226498077No Hit
TGTCTAGACTACACATTGGGTACAGTGTACACTGCTCAGGTGATGGGTAA20.10995052226498077No Hit
ACTTTATATTCTTTGTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAA20.10995052226498077No Hit
GTCAAATTGTTCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATTTGA20.10995052226498077No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC20.10995052226498077No Hit
CTACTAAGCACGTGGCATCCTCCCAGATAGAGAACCAACTCATTCACACA20.10995052226498077No Hit
ATGTAAGTGAGATCATGCGATATGTTTCTTTCTGTGTTTGGCTAATTTCA20.10995052226498077No Hit
GATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA20.10995052226498077No Hit
TGCTATCCTCGATATCCTGGTGTCAGATGTGTCTGCAGAGACAACTGGAA20.10995052226498077No Hit
CTGTGAAAGGAGGGGAGAGTGTTCACACTCAAGTGAGGAAAATGGAACTT20.10995052226498077No Hit
GGCATTAGCATGAACCAGTTTCTTGACAATTCCTCAATAGTCATGTCAGT20.10995052226498077No Hit
AAATGGAATTTGAACCATTTCAATCTTTAGTCCCCAAGGCCATTAGAAGC20.10995052226498077No Hit
GTCATGTCTTTCTTTTATTATTTGCCTTTGTGTTGCCGCCAGTTTTAGGA20.10995052226498077No Hit
CAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATG20.10995052226498077No Hit
TTTATATGCAGCTTCTTAAAGCCAGAGAGCCAGAGAACTCAGACCTTGGT20.10995052226498077No Hit
AGTGAATGCTGCCATTGTGGTTGACTTTTCAAATGGGAGGTTTCTTTGTA20.10995052226498077No Hit
ATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTG20.10995052226498077No Hit
GGTCTCAACACCTGCTTCGCGATACATAACTGACATGACTATTGAGGAAT20.10995052226498077No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)