FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_P1_POSCONT_HA_PB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_P1_POSCONT_HA_PB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284053
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT94780.4149641010957277No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA61250.26816365469627895No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG58730.25713063576020345No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC58700.25699929029667873No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT53450.23401383417985486No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT47770.20914575975251012No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG44880.19649281343296324No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT43940.1923773222425224No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT42000.18388364893459128No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA41060.17976815774415042No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG38000.1663709204646302No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT37550.1644007385117596No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA35390.15494386513798059No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC34410.15065324666284013No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT33590.14706313732649812No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA32730.14329790070545648No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT32380.1417655369643349No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT32290.14137150057376077No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG31590.1383067730915176No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA31310.13708088209862032No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA31310.13708088209862032No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA31090.13611768203277247No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG30560.1337972455105026No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT30450.13331564547757868No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT29690.12998822706828608No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT29310.12832451786363977No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC28760.12591651769902013No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG28420.12442793577907343No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC28170.12333339024970086No Hit
GATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA28060.12285179021677692No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT27680.12118808101213062No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT27190.11904277177456041No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC27130.11878008084751099No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA26800.1173352807487392No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC26560.11628451704054153No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG26330.11527753515351877No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT25710.11256306224067479No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT25390.11116204396307791No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA25210.11037397118192967No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC24980.1093669892949069No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC24690.10809731648083473No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT24370.10669629820323784No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT24160.1057768799585649No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT23990.10503258899859154No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA23940.10481367989271702No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG23880.1045509889656676No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC23540.10306240704572092No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT23310.10205542515869816No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT23080.1010484432716754No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG22990.10065440688110128No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC22980.10061062505992636No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT22920.10034793413287696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAC2100.069.0448763
TCGGACA2150.067.439184
GACATCT2350.061.6996777
CGGACAT2600.055.7670175
CATATAC10650.053.0964853
TACTGAT11700.047.0921487
CTTCTAA18750.046.7858351
ACTGATG11750.046.2747578
TCCTCCC951.9225008E-645.78766145
TATACTG23150.044.782245
TTTCGGA3400.044.7776342
TTCTAAT20050.043.3897972
TAGATGG19800.042.473068
CTTTCGG3500.041.4278341
TATGTTT1953.6379788E-1240.895813145
GTATAAC750.001483915338.665981
GAGTGGC1901.2914825E-1038.1563846
AGTGGCC1951.70985E-1037.1780137
CCAGAGT1951.70985E-1037.1780133
AGAGTGG2002.237357E-1036.248565
Produced by FastQC (version 0.11.5)