FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936876
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA113110.3851371321090846No Hit
GTAGTAACAAGAGCGAGGCTAGTTGAAATTTATGGGTGAGGAATATGTTG62620.2132197614063379No Hit
ACAACAGCGAGGCTAGTTGAAATTTATGGGTGAGGAATATGTTGCACCAG60510.20603525651065963No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC60000.2042987174126521No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA53410.1818599082834958No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA43740.14893376499382338No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT41760.14219190731920586No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG41640.14178330988438054No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG35410.12057029305970017No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT33900.11542877533814844No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG29900.10180886084397163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA114250.098.1064151
TAGTAAC124950.089.4135062
AAGAGCG29000.088.999759
GTAACAA126550.087.8824
AGTAACA132150.084.432213
TAACAAG133100.083.3938145
AGCACGC20500.078.51331
GCACGCA20700.078.103642
CACGCAC22750.071.065733
ACAAGAG161900.068.514317
AACAAGA166600.066.711996
AACAGCG16700.065.55373
ACAGCGA16850.065.4004064
CAAGAGC158850.062.846848
CGCACTG2700.061.759085
ACAACGC18050.060.6507958
ACGTGCA14200.059.225182
CAGCGAG16100.058.990515
AGCGTGC5700.055.964758
AAACACG38000.054.9472055