FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995696
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA97570.3257006051348334No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA42990.1435058831069641No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT41000.13686301947861199No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC39800.13285727256704286No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT36280.12110708162644007No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT31470.10505071275590046No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA30870.1030478393001159No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC30850.10298107685158975No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG29970.10004352911643906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA116300.0112.4609151
TAGTAAC122800.0106.386542
GTAACAA133900.097.6215064
AGTAACA140700.092.851943
TAACAAG142350.091.4191745
AGCACGC36950.078.485671
GCACGCA38450.075.609852
CAAGAGC155250.075.511998
ACAAGAG174250.074.683047
AAGAGCG32650.073.277119
AACAAGA189300.068.745486
GCCGACC2100.065.59517145
CACGCAC45100.064.782673
ACGCACT50000.061.9139024
CGCACTT43550.061.42835
ACGTGCA22000.059.6466672
CGGTGCA7450.059.361372
AAGAGCC82400.058.0703289
AAACACG58450.057.428415
GCGAGCG400.006052484354.374043
Produced by FastQC (version 0.11.5)