FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741782
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA79250.28904559151675807No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA67620.2466279230077373No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC52380.19104363512489322No Hit
GTAGTAACAAGAGCGAAACTCCATCAAAAAGAAAAAAAAAGAAAGAGAGA47980.17499567799336344No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA44630.16277734699549415No Hit
GTAGTAACAAGAGCGAAACTCCGTCTCAAAAACAAGAAAAAAAGAAAAAG39660.14465044996283438No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC35260.1286024928313046No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG32260.11766070387798884No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT32070.11696772391094551No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA30040.10956378005253517No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG29280.10679186018436185No Hit
ATTATGTACTGAGTAAACACTGGGAAGAGGCATGCCTGCCTCCCAGACTG27880.10168569200614781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA109950.0121.790151
TAGTAAC124850.0107.1953052
GTAACAA132450.0100.7159964
AGCACGC25000.099.760581
AGTAACA138400.096.6483
TAACAAG138900.095.621565
GCACGCA26300.094.5520552
ACAAGAG158900.083.586137
AAGAGCG35350.081.421979
CAAGAGC163100.079.655668
CACGCAC32000.078.163093
AACAAGA171300.077.4508746
AAACACG54850.069.5251165
CGCACTT37250.064.032765
ACGCACT42950.063.2995644
AAGAGCC96200.058.708769
GAAACAC67200.056.316264
AACAACG21100.056.0064137
AAGAGCT46600.055.853339
GTCTCGC8350.055.5691871
Produced by FastQC (version 0.11.5)