FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287675
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG1010213.0727185625099804No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG325850.9911259476681848No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA247020.7513516390762469No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT234850.7143345981582729No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT226260.6882067114298098No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA218210.6637213228193175No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG167670.5099956656299665No Hit
GAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGCATGAG150340.45728364269582605No Hit
TCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTGTA142850.43450158546693335No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT118590.36071083668549964No Hit
GATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTTCAAGA105910.3221425475449976No Hit
ATATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAGGGAGTAAGAAG105180.3199221334225555No Hit
GTTCAAGACACTGTGAAAGGAGGGGAGAGTGTTCACACTCAAGTGAGGAA103480.31475130601412854No Hit
GTTAAAAATCCAAGTAGTACCTAGCGCAAGTTCAAGACACTGTGAAAGGA103240.31402130685058594No Hit
AACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCCA94940.28877550244473676No Hit
ATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCCTAGAG94790.28831925296752264No Hit
CCCTAAGGAAGAGGAAGAATATGATTTTCAGACTTGTCATGTTATGTTAC90000.2737496863284844No Hit
GCCCTAGAGTCAGAAAGAATAGGTACACGTTTCCTTCAACATTTTCAAAG87640.2665713612203153No Hit
GTCTAATATCTTCTTTAATTTTGAAGAATAGCTTTACTGGTGCAGAATTC85160.2590280365303748No Hit
CTTTATATTCTTTGTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAA84730.25772012136236094No Hit
ATATTAGACATTTCCATATTTTTTGAAAGCAGAACTTGCAGACTCGTCAA84130.25589512345350435No Hit
TGTATACACACACATATAAATATATCCTTTTATCATATCCTGGCAAACTT83600.25428304196734774No Hit
CTTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAG77800.23664139551506763No Hit
GTGAAAGGAGGGGAGAGTGTTCACACTCAAGTGAGGAAAATGGAACTTGA77770.2365501456196248No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT76740.23341723254275437No Hit
GTAGTACCTAGCGCAAGTTCAAGACACTGTGAAAGGAGGGGAGAGTGTTC75930.23095348536579802No Hit
CCCAAATACAATGCAATTTTATTAATGATAGTTCTCATGTTGGACATGAA74910.22785098892074188No Hit
ATCATATCCTGGCAAACTTGAGCCTGAAGAGTTTAGAAATATATTGATTC72370.22012516443991573No Hit
GTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCCTAG71740.21820891663561637No Hit
CTGTGAAAGGAGGGGAGAGTGTTCACACTCAAGTGAGGAAAATGGAACTT71710.21811766674017355No Hit
CAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCCAAA69170.21039184225934743No Hit
ACTTTATATTCTTTGTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAA68420.2081105948732767No Hit
GTATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAA68020.20689392960070566No Hit
AGAAATAACAGAATGGAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAA66580.20251393461944994No Hit
AAATAATGTATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTA66180.2012972693468789No Hit
ATGTATATGTATACACACACATATAAATATATCCTTTTATCATATCCTGG64570.19640019162478042No Hit
TTCTTTGTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAA64330.1956701924612378No Hit
TCTCTCTTCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGAC62050.18873520040758285No Hit
AAGTAGTACCTAGCGCAAGTTCAAGACACTGTGAAAGGAGGGGAGAGTGT61150.185997703544298No Hit
GTATATGTATACACACACATATAAATATATCCTTTTATCATATCCTGGCA57370.1745002167185017No Hit
GAATAGGTACACGTTTCCTTCAACATTTTCAAAGAATCAGCTCATGCACA56900.1730706350232307No Hit
AATGTATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAA56390.17151938680070264No Hit
CCCTATCTCTGTATATTTAAATTTTTAAGAAGATTCCACATGTAAGTGAG55680.16935980594188904No Hit
GAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGC55510.16884272320104632No Hit
CTCTAGGGCAGCTTCCTCATTTCTTCTTTCCAATTAGAACTCCTCTGCAT54340.16528397727877603No Hit
ATTTATATGTGTGTGTATACATATACATTTTAAATATATAAGTAATATAA53600.1630331465245196No Hit
TCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAG52600.159991483343092No Hit
GAATGGAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGC52190.1587444014387067No Hit
CTTCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTG51400.15634148752537885No Hit
ATTTTAAATATGTTTGAAGAATTAAAGAAACCATGTCTTTAAATGTCTTA50720.1542731565620081No Hit
ATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTTCAAGAC48690.14809858030371006No Hit
GTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCCAAACACGCCTC48350.14706441482202467No Hit
GTATACACACACATATAAATATATCCTTTTATCATATCCTGGCAAACTTG48160.14648649881755343No Hit
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA47560.1446615009086969No Hit
CTTTATATTACTTGAAGTTTACTGGGCTTCTTGGGTGCATAATGTTTTAA47210.1435969187951972No Hit
ATATAAAGAGATCCATATCTAGACACATTTAAATCAAAGTGTTGAAATAC46910.14268441984076893No Hit
AAACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCC46420.1411940048818694No Hit
AATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAGTTTAGA46360.14101150509098376No Hit
GTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCC45660.13888234086398443No Hit
GTAGAAACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAA45490.13836525812314174No Hit
TATTAGTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCA44240.13456317914635724No Hit
GCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTGTAGCTTGCTTAA42700.1298790178469587No Hit
TTTTAATGCTGAATGATATTCCTGTGTGTGTGTGTGTCTTTGTGTTGACA42670.12978776795151586No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG42510.12930110184248747No Hit
AGTAGAAACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCA41730.12692860456097393No Hit
CAAATATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAGGGAGTAAG41660.126715688138274No Hit
ATTAGTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAA41360.12580318918384573No Hit
GTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCCTAGAGTCAGAAAGAA41060.12489069022941744No Hit
GTGTACACTGCTCAGGTGATGGGTAACCAAAATCTCAGAAAACACCACTT40920.12446485738401758No Hit
ACCTAGCGCAAGTTCAAGACACTGTGAAAGGAGGGGAGAGTGTTCACACT40490.1231569422160037No Hit
GCTCTAGAAATAACAGAATGGAATTAGGGAGTAAGAAGCAACATTTGCCC39970.12157527736166135No Hit
TATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAGGGAGTAAGAAGC39910.1213927775707757No Hit
TCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCC39870.12127111104351859No Hit
CTATTAAGCCTCCATTGTATATAAAGAATCTCTTAATTATTGCTGCTTTC39700.1207540283026759No Hit
TATCTAGACACATTTAAATCAAAGTGTTGAAATACAGAGAGAATATTGAA38670.11762111522580546No Hit
GAATATAAAGTTCTTCAAGTTCCATTTTCCTCACTTGAGTGTGAACACTC38470.11701278258951994No Hit
TTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAGA37770.11488361836252063No Hit
GTAAATAATTGTTTTACTCCCTATCTCTGTATATTTAAATTTTTAAGAAG37540.11418403583079227No Hit
ACCTATATCTTCTCATTTCCTCCCCTCTTTGACCAGTAAATAATTGTTTT37070.1127544541355213No Hit
GGCTTAATAGTGTTAACAGCTGACTTCTCATCAGAAACCATGAAATTTAG37030.1126327876082642No Hit
GTTCACACTCAAGTGAGGAAAATGGAACTTGAAGAACTTTATATTCTTTG36370.11062528990852197No Hit
AAATAACAGAATGGAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGG36050.10965195769046515No Hit
TATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAGTT35950.10934779137232238No Hit
ATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAGAAACCA35640.10840487578607982No Hit
GTCTAGATATGGATCTCTTTATATTACTTGAAGTTTACTGGGCTTCTTGG35040.10657987787722327No Hit
GTTAACATACAGTGTCTGAGGAAGCCCAGATATTGGACACACTAGACAAA34940.1062757115590805No Hit
GTATAACCCATAACAACAACTACCAGAAGTTAACATACAGTGTCTGAGGA34920.10621487829545195No Hit
CTTTAAATGTCTTACAAAATAGAAACTTTCAATAAAGAGATAGAAACTGC34870.10606279513638056No Hit
TACCTATTCTTTCTGACTCTAGGGCAGCTTCCTCATTTCTTCTTTCCAAT34090.10369029785486704No Hit
GTTCTCATGTTGGACATGAAATCTGACTTGTTTATCCCACATATCTGCTA33470.10180446668238194No Hit
ATGTATCATAGGAGCCTCAAATGGAGAACAGAGAAATAACTGGGAAGAAT33100.10067905130525372No Hit
ATGTAGGTGAATCTGGAGGACATTTGGCTAAGTGAAATTAGCCAAACACA33100.10067905130525372No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGC100.007091001145.004061
GTAGTAA89200.0104.282621
TAGTAAC90150.0102.857312
AGTAACA110800.083.68763
TAACAAG111550.082.734985
GTAACAA112050.082.689314
AAGAGCG29250.072.375899
ATCTTCA228250.066.003217
ACAAGAG138800.065.86527
CGGTTTA1009.094947E-1265.24985145
CAAGAGC141300.064.5469068
TGACTCC10400.062.04312145
AAGAGCC99200.059.0522779
CACGATA502.0110985E-457.9989782
GACTCCC5800.057.49986145
TGACTCT208450.056.657337145
CGAGCCG651.0943122E-555.768255
TAGTACA1450.054.9990272
TGACGCC2500.052.19987145
AAGAGCA18300.051.502619
Produced by FastQC (version 0.11.5)