FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874985
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA88280.30706247163028677No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA65560.22803597236159492No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA47850.16643565096861376No Hit
GTAGTAACAAGAGCGAAACTCCATCTCTAAAAAAAGAAAAAAAGAGTTGT47700.16591390911604756No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG45750.15913126503268712No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT43500.1513051372441943No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC41290.14361814061638584No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG41070.14285291923262208No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG36760.12786153666888697No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA34410.11968758097868337No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG34160.1188180112244064No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT33040.11492233872524552No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA32350.11252232620344105No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC31310.10890491602564883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC74500.097.217212
GTAGTAA76000.096.7293551
GTAACAA85750.084.293674
TAACAAG88400.081.438725
AAGAGCG22450.078.152089
AGTAACA94300.076.804693
CGGTGCA3900.076.217232
ACAAGAG114500.062.748327
CAAGAGC112850.062.4451378
AACAAGA132150.054.4773566
AAACACG35450.052.9684755
AGCACGC9550.050.1042441
GCACGCA9900.049.7975162
CGAGCAT9400.048.589973
GCGGTGC9600.048.3328821
TGACGCC4750.047.315346145
AACACGA30650.042.5770876
CAACGCA25200.041.1412059
GCGAAAG6400.040.780872
AGCATAG26000.039.8746265
Produced by FastQC (version 0.11.5)