FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259415
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA69920.2145170222263811No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA58830.18049251169304922No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA42700.13100510367658No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT37990.11655465781436239No Hit
ATATTAGACATTTCCATATTTTTTGAAAGCAGAACTTGCAGACTCGTCAA37560.11523540267195187No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG37440.11486723844616288No Hit
GTCTAATATCTTCTTTAATTTTGAAGAATAGCTTTACTGGTGCAGAATTC35650.1093754554114772No Hit
CCCTAAGGAAGAGGAAGAATATGATTTTCAGACTTGTCATGTTATGTTAC35520.10897661083353914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA108500.077.314161
TAGTAAC119100.069.395062
CGAGCTA4450.066.797365
GTAACAA124700.066.046124
TAACAAG128900.063.725385
AGTAACA140800.058.6999363
GCACGCA20650.051.2587782
ACAAGAG164350.050.421067
CAAGAGC150150.049.250468
CGTCCAG8850.048.3330465
AGCACGC25400.045.6711241
AAGAGCG20700.045.531139
GTCGTCC10050.042.561943
AAGAGCC89650.041.72879
GCGGTGC12200.041.600031
AACAAGA209050.039.7785266
CACGCAC26600.039.5204433
CGAAAGC7950.039.2136044
TCGTCCA11750.036.4040374
GCAAAAG35500.034.3096583