FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652115
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC231150.8715685405798769No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG87840.3312073571470317No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA86090.32460884991789574No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT83290.31405123835127813No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT73800.27826847629156354No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT72110.2718962035959979No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT69680.26273370498639764No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA69110.26058447691747905No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT64110.24173159911994768No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA58030.2188064997181495No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC57760.21778844431708277No Hit
GTATAAAGGAGTAAAAAACTGTTCTCAAAGACTCAATGCCATCATTACAT55110.20779641908439112No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT53610.20214055574513173No Hit
AGTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAAT53130.2003306794765687No Hit
ATATATTTCTTAATCTGTAATGATAATGTGATTTCCAAGCATTTCATCAT53050.2000290334318082No Hit
ATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCT51840.1954666370048056No Hit
TTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAGATTGCT51210.19309117440231666No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA51110.19271411684636602No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC50760.1913944154005388No Hit
TATATATTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAG47260.17819740094226683No Hit
TATTTATATAGTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGG47030.1773301685635804No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC47000.17721705129679519No Hit
GTACTAAATATATTTCTTAATCTGTAATGATAATGTGATTTCCAAGCATT45710.1723530088250321No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC44320.16711190879731835No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC41330.1558378878743946No Hit
TTCTTAATCTGTAATGATAATGTGATTTCCAAGCATTTCATCATTTGGAA40810.1538771885834513No Hit
GTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATAAGTTTATTGCC39220.14788197344383633No Hit
GGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACTTAAAATCCAAGG38880.1465999777536042No Hit
GATTATGTCAGTCATTGTAGGAAGAAAAATAAGTTTATTGCCGCATTATT38570.14543109933015727No Hit
ATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATAAGTTTATTG35500.13385543236247296No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC35280.13302590573938158No Hit
TTTATATAGTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAA34730.13095208918165313No Hit
GTAGAAACACGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC34520.1301602683141568No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC34420.12978321075820617No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT34260.1291799186686852No Hit
GTAATGATAATGTGATTTCCAAGCATTTCATCATTTGGAAGAGTTGGAGA34230.1290668014019No Hit
ATCATGTAGTTGTACTAAATATATTTCTTAATCTGTAATGATAATGTGAT33980.1281241575120234No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA33930.1279356287340481No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT33690.1270306905997666No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA33580.1266159272882209No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33520.12638969275465053No Hit
CTTCTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACA32600.12292076323990475No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32500.12254370568395413No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG31660.11937642221396885No Hit
CCTTTATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGTCTCAAA31270.11790589774576139No Hit
CTGTAATGATAATGTGATTTCCAAGCATTTCATCATTTGGAAGAGTTGGA31170.11752884018981077No Hit
CAATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAA30330.1143615567198255No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA29870.1126270919624526No Hit
GATCTATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGC29850.11255168045126247No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC29310.11051556964912909No Hit
GTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA28970.10923357395889695No Hit
TCTTAAAATTCAGTAGGTAGTGGCGATTTTGCATGTGAAAACAAGGGTGG28540.10761222646830926No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA28520.10753681495711913No Hit
GTAGAAACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAA28110.10599087897772154No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC27430.10342688759725728No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT26600.10029730988286706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA81300.0106.11764
TAACAAG84750.0101.541145
TAGTAAC90950.095.495992
AAGAGCG44250.086.99869
AGTAACA100550.086.522733
AACAAGA110250.078.055436
CACGCAC2550.076.7634663
AGCACGC2550.073.9231641
GCACGCA2650.073.866742
AAACACG28500.073.516365
TGACGCC1450.069.99887145
ACGAGCG9900.068.1049659
ACACGCC1300.066.922005145
GTAGTAA132700.066.764821
CGGTGCA2500.066.698922
GTCTCGC808.092684E-963.4388661
ACAAGAG137250.062.5946167
AACACGT9800.062.1418536
ACGTGCG3700.060.7422689
GTAGAAA58700.060.52091
Produced by FastQC (version 0.11.5)