FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399528
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG189280.7888218016209856No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG91600.38174174254270005No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA77470.32285516151509797No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA42580.17745156547454333No Hit
GTGTTTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTA40030.16682447548017776No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA38570.16073994552261944No Hit
GTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTA36210.15090467791999093No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA35150.1464871424713527No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT34590.14415335015886457No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT34110.14215295674816047No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG32000.133359560713607No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC28640.11935680683867826No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG26910.1121470555875989No Hit
GAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGCATGAG25160.10485395461107352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA92350.0102.291264
GTAGTAA93100.0101.936571
TAGTAAC93600.0101.0026552
AGTAACA96000.098.55313
AGCACGC10300.092.2091751
CGTGCAC7150.087.201393
TAACAAG109150.086.879045
CGAGCCG3000.079.748725
CACGCAC12150.078.764163
AACAAGA123250.076.469356
ACAAGAG129450.072.750867
CAAGAGC123350.072.4694448
ACGTGCA14000.070.945292
GCACGCA14050.069.144792
GATAGCG2250.061.222511
AAGAGCC74100.060.4658749
AAGCGGT2600.058.556752
ACGCACT20100.057.349824
CGCACTT15650.056.5166635
ATCTTCA49400.056.208617
Produced by FastQC (version 0.11.5)