FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069469
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCCAGAGTCAAAACACCTTCTTGTATTTGCCAAACATTCATTAA68390.22280726731561712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGCG3900.037.17906145
CGTGCAT13500.033.8323973
GTAGTAA365300.032.865231
CCGACTG6200.032.7415669
ACGTGCA23300.032.359622
AAGAGCG20200.032.3016139
TAGTAAC371500.032.1996462
TAACAAG376150.031.5900975
ACAGCCA44950.031.4507434
GTAACAA382350.031.1342264
AGTAACA389250.030.7872033
GCCAGAG33400.029.0864967
CGATTCA7450.028.221155145
ACAAGAG408050.027.8945487
GCCGACT7300.027.8079058
AACAAGA410700.027.6262996
CAAGAGC368150.027.5699718
CCCTAAG14050.027.3481
GGACCAT12500.027.2596918
GCATAGA13200.025.813681
Produced by FastQC (version 0.11.5)