FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686410
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA110090.6528068500542572No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG67210.39853890809471004No Hit
ACAACAGCCACTCTTCTTCCTTCCGTTTTTAAGTTATAAAAAGCATTTAA50470.29927479082785324No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG42750.2534970736653602No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT42630.2527855029322644No Hit
GTAGTAACAAGAGCCACTCTTCTTCCTTCCGTTTTTAAGTTATAAAAAGC39110.23191276142812248No Hit
TCCATATACAGAATCTCAAATGATTCTAAAAATGGAGCCCTTAGGTAATG31500.1867873174376338No Hit
AACACGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC30770.182458595477968No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG28990.1719036296037144No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT28460.16876085886587483No Hit
TTCTAATGCATAGCCAAAGTCAAGAATCACTAGAGCTCTCTTTAAAATTC28000.1660331710556745No Hit
AACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC26600.15773151250289075No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG23470.13917137588130998No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA23080.13685877099874882No Hit
AGTTCACACACTGCCTGAACATTATAATCGCCTAGGGAACAACACACACA22330.1324114539169004No Hit
GTAGTAACAAGAGCAGGGGGAGGAGGTAACGATTGGTTCACTGCTTAATT20360.12072983438191187No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT20060.11895090754917251No Hit
CTTCATAATCAGAACAAACATGTGATTGAGTGGAACAAGCTAGTGCTAGC19720.11693479047206788No Hit
CTTTAAAATTCATATGTGACATATCCTTTTAACCCCAAAACAACCACACT18070.10715069289200134No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT17490.10371143434870524No Hit
GTATATGGAAGAGTATTTTGGAAACTCTGATGTGCTATTGAAATAGAAGT17490.10371143434870524No Hit
CTCCTTGACACCTGACACAATACCTTCCCACAATAGCCACTTCATAATCA17110.10145812702723536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA68900.0107.328961
TAGTAAC72200.0102.222512
TAACAAG73200.099.3403855
GTAACAA75950.096.220754
AGTAACA85050.087.1189353
AGCACGC4450.079.8310851
GCGGTGC4500.077.332971
CGGTGCA2300.075.651822
GTCTCGC2950.071.270851
CGAGCTA7000.067.321115
CAAGAGC101100.067.193068
GCACGCA5200.066.922762
ACAAGAG108400.066.814727
AACAAGA110400.065.8013156
ACGTGCA11000.065.2496952
AAGAGCG5450.062.5226449
AAGAGCT42500.061.0702979
CGCTCTG3450.060.9417425
CGAGCAC4850.056.8038563
CGTGCAC5100.055.4409183
Produced by FastQC (version 0.11.5)