FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932895
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG86210.4460149154506582No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA82730.42801083349069663No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA50020.2587828102405976No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT42170.21817015409528193No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT39730.2055466023762284No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG36900.19090535181683432No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG34740.17973040439340987No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC34390.1779196490238735No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG33290.1722287035767592No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC28830.14915450658209578No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA25790.13342680280097988No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC25280.1307882735482269No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC24980.12923619751719573No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC24510.12680461173524687No Hit
CTTAAAATCTACTCTTTTAGCAAAAATCCTGAATACAAATAATTTTATTA24440.1264424606613396No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC24360.12602857371973128No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT23600.12209664777445231No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG23310.12059630761112217No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA23300.12054457174342113No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA22470.1162504947242349No Hit
GTAGTAACAAGAGCAAACCCTAAGGAAGAGGAAGAATATGATTTTCAGAC22460.11619875885653386No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG22300.11537098497331723No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT21050.10890400151068733No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA19720.10202313110644913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC68900.094.806852
GTAGTAA73350.089.05741
AGCACGC8450.084.084271
GCACGCA8400.083.719492
GTAACAA78750.082.856414
AAGAGCG12000.082.7700969
TAACAAG79200.081.653315
AGTAACA84150.077.453263
ACAAGAG98550.065.6209267
CAAGAGC92800.065.233798
CGCACTG2650.057.4523165
AACAAGA117200.055.240556
CGGTGCA2600.052.9802972
CACGCAC14000.052.3031043
ACAACGC9150.052.2946178
CTAACCG604.946452E-448.33294
TAACCGG604.946452E-448.33295
GTAGAAA62000.047.4766161
AAGAGCC65300.046.7415439
TAGTAGT12950.046.4667664
Produced by FastQC (version 0.11.5)