FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088542
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA123440.3996707831721246No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG67650.21903538951388712No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA63800.20656996084236512No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG54020.17490453424301824No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT51940.1681699649867154No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA47630.15421516042197259No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT45700.14796625721780698No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG43650.1413288211719316No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT37700.12206406777048846No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC37450.12125462435025977No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG33490.1084330405738371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA109450.0117.512441
TAGTAAC117200.0109.303452
GTAACAA132300.096.663764
AGTAACA135400.094.557713
TAACAAG140650.090.822015
ACAAGAG164000.077.714137
AAACACG44450.076.330665
CAAGAGC160550.076.135338
GCACGCA22900.074.7139052
AGCACGC23900.071.894631
AACAAGA187150.068.139816
AAGAGCG24850.066.5192649
GTAGAAA74500.066.273031
CACGCAC28250.063.130843
ACAACGC15150.061.2542658
GAAACAC55850.060.620384
AAGAGCC83750.059.9046139
CGCACTT26950.057.567845
ACGCACT33550.057.263554
ACGTGCA25400.054.80152
Produced by FastQC (version 0.11.5)