FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluB_102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluB_102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265941
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA203180.6221177908602757No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG184120.5637578878491681No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA94040.28794151517127836No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG93780.287145419957066No Hit
AGTAGAAACAACGCACTTAGCCGATAAAGAATTTTTTGGCTAGAGGGCTT84340.2582410398718164No Hit
GTCTGAGGGTGTCGGCAAACAGAGCAGACAGTAGGCTTGTGTGCAGAACG81390.24920842109517594No Hit
ATTATGTACTGAGTAAACACTGGGAAGAGGCATGCCTGCCTCCCAGACTG75100.2299490407205764No Hit
ATTCAAGGGCTTCCCCAATTTACCAGATGTCAAGGTCGTATGTAATGTTC61160.18726608962011254No Hit
GAGTAAACACTGGGAAGAGGCATGCCTGCCTCCCAGACTGGACCTGGGTC61030.18686804201300636No Hit
GTATATAGCTTTATAAGCCTTTTCCAGATGAGGATTTCTTTAAGTTCTCA57530.17615137566783967No Hit
GTGTAAGTACCGCCAGCCTGGCCATATGGGCCTCTGAGATATGGGAGATG53300.16319951891353826No Hit
CCCTCAGACTGTGGACTGTGTGTTTACCCTGGTTACTCCCTGGCCCAGCC52710.16139299515821015No Hit
CAGTTATACTTGAGCAAGTGAAGGGAAAAACTTTGCTGCCTCTTCCAGCA50900.15585094770542396No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA48110.14730823367599108No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA45330.13879613869325869No Hit
ATCCTGATAAGCAAAATACGGAAAGCTAGTGTGGTTGCGCTCAGCCCTGA44340.13576485306991154No Hit
GTAATAGCCTTGGAAGACTTCATGCAGTGGCTGGGCCAGGGAGTAACCAG43260.13245799602626013No Hit
GTAGTAACAAGAGCGAAACTATCTCTCAAAACAAAAACAAAAACAAAAAC43040.13178437699884962No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT41900.12829380567499535No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC41420.1268240914333725No Hit
ATTATTAGGCCTTCCAGCACAGTGACCTGCAAAAGGTTGTATATAGCTTT40620.1243745676973344No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT39890.12213937728819964No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT38790.11877128215114724No Hit
TTATTAGGCCTTCCAGCACAGTGACCTGCAAAAGGTTGTATATAGCTTTA38430.11766899646993009No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA38320.11733218695622488No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG38030.11644423460191106No Hit
TTATAAGCCTTTTCCAGATGAGGATTTCTTTAAGTTCTCAGAATTGGAAC38030.11644423460191106No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT37160.11378037753896962No Hit
ACAACAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTAAACACT37050.1134435680252644No Hit
GTATAACTGAGAGGTCACATTGGAGGCTGTGGGCGTGCCGCCTGACATCC37050.1134435680252644No Hit
ATATGTGAGGATGTCAGGCGGCACGCCCACAGCCTCCAATGTGACCTCTC36960.1131679966049601No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT36810.11270871090445296No Hit
CATGATATGTGAGGATGTCAGGCGGCACGCCCACAGCCTCCAATGTGACC36000.11022856812171439No Hit
GTCCTGGCCAATGAGAAGAATCGCCTAAACTGGCCCCACATGATATGTGA35900.10992237765470962No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC34830.10664613965775867No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC33940.10392104450141629No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC33820.10355361594101056No Hit
TTCCAGCACAGTGACCTGCAAAAGGTTGTATATAGCTTTATAAGCCTTTT33060.1012265683917744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA126350.0113.613161
TAGTAAC128500.0111.3722
AGTAACA138800.0103.15963
GCACGCA17850.097.477662
AGCACGC18600.094.328111
TAACAAG151750.094.2128755
GTAACAA153900.092.849614
CACGCAC21800.080.480553
CGAGCCG7500.078.298935
AACAAGA185050.077.1807866
CGGTGCA3550.071.47792
ACAAGAG199700.071.373587
CAAGAGC195150.069.806678
AAGAGCG27750.067.928039
ACAGCCG9600.066.457424
ACGTGCA12850.065.446572
ACAACGC25350.064.92128
AAGAGCC117600.063.4376039
AACAACG27250.060.6597187
AAACACG45200.060.308915
Produced by FastQC (version 0.11.5)