FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_MK1069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_MK1069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777508
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA244820.881437605220219No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT143520.5167221840585158No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA142840.5142739462856633No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG132100.4756061908732576No Hit
GTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC115630.4163084318749037No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT99210.35719069035984774No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT97380.35060205047114174No Hit
GTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAAC95200.3427532881993499No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTC93570.33688471824383587No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC91580.3297200224085763No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT89790.3232753965065087No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAG84680.3048776097134554No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA83080.29911705024792007No Hit
AAGTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTT80660.29040420405629797No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA75850.2730865221630325No Hit
GTCTTATTGTGTTGCCCAGGCTGTGTTACTATTATCACCTGAAAGGTTAT74810.2693421585104345No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCT72750.26192543819855785No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTT71600.25778503608270437No Hit
CTATTACTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTG68360.24611990316499538No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT65520.23589491011367023No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT65500.23582290312035106No Hit
CCCATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCG62900.2264619939888562No Hit
ACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGG61670.22203356389972595No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT59910.21569694848763712No Hit
GTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCAG59280.21342872819808262No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT58390.21022441699537858No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA57150.20575998340958873No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGC57110.20561596942295035No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC56190.20230364773026754No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA50930.1833658084873203No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC50790.18286175953408595No Hit
GAATAGGAGTGCCTTTTTTTTTCTTTCTTCTCCCTTTTCTTTTCTACTTC50030.1801254937879567No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCC49050.17659715111531632No Hit
ATTCTGGACAGTGTTCATTGCCCAATCAGAACATCTCAATCCAAAACGGG47250.17011652171658911No Hit
GTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCAGACCCACCATT46400.1670562245005235No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA44640.16071960908843466No Hit
AGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAG44220.15920746222873164No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT43150.15535508808615492No Hit
CCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA43060.15503105661621858No Hit
GGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACA42260.1521507768834509No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC41950.15103466848700348No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA41310.14873044470078933No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT41290.14865843770747014No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA40640.14631821042459645No Hit
ATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAAC40220.14480606356489342No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA40010.14404999013504194No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG39740.14307789572523283No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT39350.1416737593555086No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA39020.14048564396574195No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA38960.14026962298578438No Hit
CTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGG37930.13656126282984604No Hit
GTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCAT37460.13486909848684506No Hit
GAGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA37440.13479709149352587No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGTAATAGTGACTAAG37390.13461707401022788No Hit
CCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGC37390.13461707401022788No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC36800.13249286770731172No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA36280.13062068588101278No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC35770.12878450755137338No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA35740.1286764970613946No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA35740.1286764970613946No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT35600.12817244810816025No Hit
GAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAG35480.1277404061482451No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC35290.1270563397117128No Hit
GTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGG34910.12568820683864818No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT34860.12550818935535019No Hit
GTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCT34460.12406804948896637No Hit
TTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAAT34030.12251989913260376No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGAACT34010.12244789213928457No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA33930.12215986416600781No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG33900.12205185367602901No Hit
GTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTC33860.12190783968939063No Hit
CTTTTTAATGACCGGCTCTCACAATAACTCATGAAAACTCACTATCAGGA33520.12068372080296437No Hit
GTGTTACTATTATCACCTGAAAGGTTATAATTAAGAAACTTATTCTTAAT33500.12061171380964518No Hit
GGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCTAACC33470.12050370331966641No Hit
GTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTC33390.12021567534638963No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT33290.11985564037979368No Hit
CGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTC33260.1197476298898149No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT33090.11913557044660177No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT33040.11895555296330379No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC32250.11611127672719575No Hit
GGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAA32070.11546321378732302No Hit
GAATGGACCAGGCGATCATGGATAAGTAATAGTGACTAAGTAAAGGGCTT32050.11539120679400383No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT32010.11524719280736545No Hit
GACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAA31820.11456312637083313No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT31180.11225890258461903No Hit
ATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGTAC31090.11193487111468266No Hit
AGTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTTG30990.1115748361480867No Hit
GTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGAA30940.11139481866478873No Hit
CTCCAGCACTGGGGATTACAATTCAACATGAGATCTGAGTGTGGACAAAT30370.10934261935519178No Hit
GGGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG30350.10927061236187259No Hit
CATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCA30350.10927061236187259No Hit
GTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCTTC30070.10826251445540391No Hit
CCCTAAGAGGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACA29890.10761445151553119No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA29830.10739843053557362No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA29490.10617431164914735No Hit
GTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCA29430.10595829066918978No Hit
GTCCAGAATAAACAGAAATCCATTTTGCACTAAGTGGCTGCACAGACCCT29130.10487818576940193No Hit
CTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCT28850.10387008786293325No Hit
GGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACC28540.10275397946648579No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC28370.10214192002327266No Hit
ATCAGGAAGACAGCACTAAAGCACAAGGGATCCGACCCCATGATCCAAAC28160.10138584659342115No Hit
GGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCGAACCAA28070.1010618151234848No Hit
AGACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCA28020.10088179764018682No Hit
CTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGG27870.10034174519029289No Hit
ATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCT27850.1002697381969737No Hit
ATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACG27840.10023373470031409No Hit
CACAATAACTCATGAAAACTCACTATCAGGAAGACAGCACTAAAGCACAA27810.10012572421033532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7000.0132.575641
AGCGAAA29500.0109.122111
AGCAAAA23750.0103.789042
GCAAAAG29950.082.0614243
TATCAAA2150.074.18573145
TGACGCC2950.071.27089145
CTTATTG17600.068.380393
CAGAAAA3750.065.733055145
TATTAGT3250.064.694371
CTAATAA4900.062.1425939
ACCGACC3650.059.58879145
ATTAGTA5600.059.5554661
AGCAGGG23400.059.17717
GCGAAAG57150.056.578942
CGAAAGC57550.056.059713
GAGCAAA45550.055.07311
TCTCGGG4100.054.81684145
ACTAATA5700.053.4208268
AAGCAGG74650.053.221486
ACATCTT3550.053.09837145
Produced by FastQC (version 0.11.5)