FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM554145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM554145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856811
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA57230.2003282681283431No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC51080.17880076770916942No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC37320.13063517327537594No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA32870.11505836402898195No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC29390.10287694915764466No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC29380.10284194509192243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11550.094.7827151
GAGCAAA66700.069.5646061
AGCGAAA19700.067.347121
CAAAAGC72250.064.220894
CGAAAGC21050.062.6835333
GCGAAAG21800.060.5269932
AGCAAAA84400.055.0617182
GCAAAAG88600.052.451573
AAAGCAG117450.049.011916
AAAAGCA99450.047.5310025
TATCGTG21600.046.654682
CGTGTAG21850.046.1208765
ATATCGT21900.046.0155751
TCGTGTA22900.044.0061654
ATCGTGT23100.043.6251563
AAGCAGG146200.039.225037
TACGACG1303.7018435E-739.0381163
CGACGGG1354.9760456E-737.592265
ACGACGG1354.9760456E-737.592264
GACGAAA6200.035.080334145
Produced by FastQC (version 0.11.5)