FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM554007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM554007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111344
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA91850.29521004427668557No Hit
TTCTTAGACAGAACCCGACAGAAGAACAAGCTGTGGATATATGCAAGGCT50180.16128078412415986No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT47330.15212075553201446No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA44810.14402136182948588No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC43250.13900745144220633No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT43180.13878246828380275No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG41850.1345077882741349No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG40030.12865822615564207No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG39400.12663337773000993No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT37810.12152304598912882No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA37560.12071953470911606No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG37520.12059097290431402No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC37420.12026956839230891No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG36660.11782689410107015No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT36580.11756977049146605No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT34540.11101311844656199No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT34470.11078813528815842No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT33450.10750980926570639No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC33370.10725268565610231No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC31920.10259232023202836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17550.093.363431
CGAATAA2500.069.59896145
GCAAAAG90900.067.155213
CGAAAGC24400.066.556384
AGCGAAA25950.062.5809482
TACGACG606.809567E-660.4157643
CAAAAGC102400.060.321364
AGCAAAA102800.060.0161252
AAGCAGG135500.057.303577
CGACGGG651.09428165E-555.7683945
GAGCAAA113700.055.1570631
TTAGACA16550.053.005254
ATATCGT7400.052.9063151
GCGAAAG31550.052.851823
TATCGTG7500.052.199222
TTACGAC701.6969705E-551.784942
TAAACTA7200.050.346475
CGTGTAG7850.049.8718645
CCGGTAC450.00964160348.332613
TACTAAT3000.045.915987
Produced by FastQC (version 0.11.5)