FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM553980.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM553980.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896083
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA124760.43078875847135595No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA54900.18956639018978394No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT54250.18732197937697226No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT53240.18383451026783418No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC51480.17775733637468263No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT50900.17575463134171224No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG47900.16539581220565847No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG47340.16346216596692845No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG42370.14630105559819936No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT42320.14612840861259846No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT42090.14533423247883434No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC41690.14395305659402718No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC41080.14184676336969623No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG40860.1410871166330523No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT40810.1409144696474514No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG39270.1355969424909438No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC37040.12789688693314383No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA36990.12772423994754292No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG36360.12554888792897165No Hit
TTCTTAGACAGAACCCGACAGAAGAACAAGCTGTGGATATATGCAAGGCT36060.12451300601536627No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG35160.12140536027455014No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC34900.12050759594942548No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA34580.11940265524157975No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA34180.11802147935677258No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG33250.1148102454245959No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA33080.11422324567355287No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT32730.1130147167743466No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA31030.10714471926391612No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT30810.10638507252727217No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG30390.10493483784822465No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGAACTAC30350.10479672025974394No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT29850.10307025040373496No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC29000.10013525164851973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12900.092.73751
GCAGGGG12600.078.829489
GCAGGGT22950.074.553499
GCAAAAG83550.070.199373
GCTGACC1200.066.458435145
CGAATAA1750.066.28582145
TAAACTA6100.065.367835
TACTAAT1900.064.867417
CGAAAGC22200.063.028293
AGCGAAA22750.061.8274771
CAAAAGC96400.061.7443584
AAGCAGG115250.061.2701877
TTTAAAC6650.061.051673
AGCAAAA99700.059.118932
AGGGTGT11550.057.748016
GCTTATA5350.055.5598752
AGCAGGT63950.054.5309688
GCTAAGC400.006052416354.3741462
CGCTCCC953.142668E-853.4202166
GAGCAAA116650.051.5886881
Produced by FastQC (version 0.11.5)