FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM553698.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM553698.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348703
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA72220.21566558754240076No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC57720.17236524110976698No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC50130.14969974942537456No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC44260.132170574697129No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA36200.10810155454216155No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT35500.1060111929902413No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA35240.10523477298524234No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA34900.1042194545171668No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT33610.10036721680005661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17950.092.0918051
CGACGGG1500.086.9986345
TACGACG1500.086.9986343
ACGACGG1750.074.570254
ATTACGA1850.070.542591
CAAAAGC96000.068.194244
GCAAAAG96450.067.500243
GAGCAAA102400.064.713791
AGCGAAA27050.060.572062
AGCAAAA111750.058.8424722
CGAAAGC28050.058.1541634
TGACGCC3250.053.538418145
AAAGCAG153900.052.7607046
ATCGACC701.696857E-551.78567145
GCGAAAG32600.051.594283
AAGCAGG163900.049.895497
CATACCG2100.048.3332949
ATATCGT24750.045.9912341
CGTGTAG25050.045.727825
TATCGTG25000.045.529282
Produced by FastQC (version 0.11.5)