FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM553445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM553445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3528127
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA55420.15708051325816788No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC44830.12706458696073014No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA35450.10047824242154549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA19500.079.936371
GAGCAAA88400.061.1824841
GCAAAAG88350.060.148623
AGCAAAA91200.058.4279632
CAAAAGC94350.057.0919884
CGAAAGC27650.055.5864524
AGCGAAA28300.054.309732
TATCGTG17600.051.0783962
ATATCGT17400.050.833631
GCGAAAG31700.050.085633
CGTGTAG18700.047.29845
AAAGCAG155550.044.0909426
GACGAAA9550.043.271893145
TGACGCC7800.042.756054145
AAGCAGG161200.042.6804927
AAAAGCA132250.041.2240265
ATCGTGT21700.040.7594533
TCGTGTA22300.039.6627854
TAGAATT45650.032.0812389
ATAAGTG45400.031.9380638
Produced by FastQC (version 0.11.5)