FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM553438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM553438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559733
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA26620.1039952213766045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA21700.0107.248671
GCGAAAG23200.0100.310562
GAGCGAA5150.098.545641
CGAAAGC25100.092.1396563
GACGAAA4450.068.42699145
CGAATAA2650.057.452847145
GAAAGCA56350.041.556434
GTAGCCG14750.037.8467258
TAGCCGT17000.032.8382459
AAAGCAG97300.032.0394675
AAGCAGG93550.031.6963446
CAAGGTA23150.030.6905524
CGTCTAT1204.0593266E-430.2089311
TGACGCC5200.027.884624145
CAAAAGC34150.027.5982994
AGGTAGC22450.026.480566
ACAAGGT32350.026.2205163
TAGAAAC23750.025.6416112
GTCGCCC4001.8189894E-1225.375006145
AAGCACG4300.025.2902095
Produced by FastQC (version 0.11.5)