FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM553323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM553323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815375
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA77630.2757359143986148No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG63210.224517160236203No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT62220.2210007547839986No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC55170.19595968565466415No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC55000.19535585845580072No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA40840.14506060471518004No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA40530.14395950805842916No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC38360.136251831461173No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG38070.13522177329840607No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT38060.13518625405141413No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG36570.1298938862496115No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT36060.12808240465302134No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT35660.1266616347733428No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC35340.12552501886959996No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA34840.12374905652000179No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC34800.12360697953203391No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT34720.12332282555609822No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT34570.12279003685121877No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT33940.12055232429072503No Hit
TCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT33770.11994849709186166No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA33700.11969986236291791No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG31560.11209874350663765No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT30460.10819162633752165No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG29890.10616702925897971No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC29650.10531456733117257No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA29110.10339652799360655No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA28710.10197575811392798No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT28300.10051946898725747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA2900.082.49907145
GAGCGAA15750.072.731921
GCAAAAG79950.061.39083
GCAGGGG10050.059.1535649
CAAGCTA4800.058.9055864
GCAGGGT30850.058.046359
CAAAAGC87100.057.35014
CGAAAGC19850.056.9766924
TAAACTA5500.056.6811835
AAGCAGG109350.055.0291027
AGCAAAA91700.054.1569672
AGCAGGG45750.052.1360248
GAGCAAA97050.051.995081
AGGGTGT14150.051.748536
GTCGCCC2950.051.609585145
AGCGAAA22450.050.3780562
TTTAAAC6700.048.693483
CGGAATT450.00964136848.332795
CGCTCCC1203.6252459E-948.332796
CTTATAA1700.046.911236145
Produced by FastQC (version 0.11.5)