FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM552916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM552916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946080
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA41550.2135061251336019No Hit
TTATAATACACTGTAAGAGCAAGACCCCTTTCTGCTGGATTCTGATAAAA24640.1266134999588917No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA22400.11510318178081065No Hit
ATAATACACTGTAAGAGCAAGACCCCTTTCTGCTGGATTCTGATAAAAGA22210.11412686014963414No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT21920.1126366850283647No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA21330.10960494943681658No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA20340.10451779988489682No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG19850.10199991778344157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7300.0108.256161
AGCGAAA23800.095.349051
GCGAAAG26200.087.1637652
GAGCAAA36100.078.92861
CGAAAGC29400.077.429963
CAAAAGC46250.060.5065354
ACCGACC606.808019E-660.41665145
GGAGCGA2000.050.751291
GCAAAAG55350.050.6897353
AGCAAAA57750.048.9597632
ACGATCC5000.043.49999145
AAAAGCA69550.040.653155
ACGCAGT1651.36788E-939.5444264
AAAGCAG94400.038.2458236
CCGGTCT2700.037.5916183
CGAACCC1406.613918E-736.24999145
GTAGCCG17800.035.8417668
TAGCCGT17850.035.7422879
GAAAGCA65150.035.6092034
GTACCGC5300.035.565115