FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM552690.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM552690.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015528
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA73860.24493223077351628No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC52690.17472893635874048No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC50820.16852770062158268No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC46920.1555946421323231No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC37500.12435633162749607No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC36470.12094067771879419No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA35760.11858619783998027No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA34990.11603274783056235No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC34360.1139435614592204No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT34200.11341297444427642No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT32940.10923460170159256No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG31310.10382924648685073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17250.0100.869011
GAGCAAA72950.079.9035951
AGCGAAA23400.073.737682
GCGAAAG24450.070.867553
CGAAAGC25350.068.065554
AGCAAAA84700.067.79072
CAAAAGC86750.066.188734
GCAAAAG89750.064.299413
AAAGCAG131600.056.5223056
AAAAGCA117450.049.2581675
AAGCAGG157050.047.5474977
TGACGCC3150.046.031487145
CGTGTAG26950.040.351615
CGACGGG1807.2759576E-1140.2768865
TATCGTG28350.039.3818442
TCACGCA11600.038.7491382
ATATCGT27900.038.7184221
TACGACG1901.2914825E-1038.1570473
ATCGTGT28900.037.879783
TCGTGTA29400.036.988984
Produced by FastQC (version 0.11.5)