FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM552654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM552654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415479
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA54560.1597433332191473No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC47820.14000964432807228No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT45810.13412467182494756No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC42440.12425782737941003No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA42010.12299885316232366No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC41990.12294029622199405No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG41190.12059801860881006No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT38170.11175592061904054No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG35430.10373361979388543No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT35190.10303093650993023No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC34480.10095216512822945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16300.085.3987661
ACGACGG1350.064.4435044
CGACGGG1400.062.1419455
GCAAAAG86700.062.046383
TACGACG1450.059.9991233
TTACGAC1450.059.9991232
GAGCAAA92350.058.957771
AGCAAAA92200.058.50242
CAAAAGC94100.058.1686484
CGAAAGC25100.055.457354
AGCGAAA25000.055.389192
ATTACGA1700.051.1764681
GCGAAAG27900.050.411453
AAGCAGG156850.043.0787437
TGACGCC3650.041.71172145
CATACCG1755.2750693E-1141.4279639
AAAGCAG163250.041.3010836
TCACGCA7600.040.0652052
CGTGTAG19200.040.0254555
TATCGTG19500.039.7814672
Produced by FastQC (version 0.11.5)