FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM552594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM552594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3097479
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA148270.4786795971820955No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC130760.4221497546876024No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA107970.3485737917835763No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC102900.33220564207214964No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC85580.2762892016378481No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC65320.21088117143005652No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT64610.20858898478407764No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA61840.19964622843286428No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA59550.19225311939160847No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC58710.18954123659918276No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG56170.18134101958399074No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC55400.17885512702426715No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC55390.1788228427053097No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT51960.16774932130290474No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA49770.1606790554512234No Hit
CTCTTTCCTCAGCCTCTTGAGTAGCTGGGACTACAGGCATGTGCCACTGT49120.15858057471898923No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA45200.14592512168766925No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG44920.14502116075686067No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT42510.13724063988811547No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA41960.13546500234545578No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT40660.1312680408809874No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT39840.1286207267264766No Hit
AGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGTGC39030.12600569689092322No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC38680.12487574572741252No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC36690.11845116625488017No Hit
GGCACGTGCCACTGTGCTCAGCTAATTTTTCTATTTTTAGTAGAGATGGG35900.11590070505724172No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG34720.11209115542026274No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT33320.10757135076621988No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG31640.10214758518136846No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC31560.10188931062970887No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC31530.10179245767283653No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG31510.10172788903492161No Hit
TCCCCACAGGCATCTTGCCTCTCACCCTGTGCTGGGCCCCGTGCTCCAGA31310.10108220265577265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA18700.0119.802451
GAGCAAA80550.0105.849941
AGCAAAA97550.086.656092
CAAAAGC104050.081.103334
AGCGAAA28500.077.58592
AAAGCAG140600.075.7983556
GCGAAAG29450.074.836953
GCAAAAG114450.074.176943
AAAAGCA114600.074.079855
CGAAAGC30900.071.325184
TACGACG2400.054.3737223
ACGACGG2400.054.3737224
AAGCAGG207350.051.8169637
CGACGGG2600.050.1911285
TTACGAC2600.050.1911282
CGTGTAG47900.049.643935
ATTACGA2800.049.197661
CACCATA48300.049.08272
ATATCGT48350.049.0343171
TATCGTG48800.048.7283632
Produced by FastQC (version 0.11.5)