FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM551963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM551963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688440
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA49170.1828941691092232No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA47560.17690556605317584No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT43530.1619154602669206No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG36530.13587805567541025No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC34960.13003823778845724No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC33230.12360327922512684No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT33210.12352888664057966No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC30230.112444391543051No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT27030.10054157801550341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16100.091.414381
CGGCATA300.001936128772.499724
GAGCAAA82150.067.602941
GCAAAAG82600.066.970083
CGACGGG1200.066.458085
AGCAAAA84600.065.1297762
TACGACG1250.063.799753
ACGACGG1250.063.799754
CAAAAGC88550.062.633864
CGAAAGC23150.062.00844
AGCGAAA23200.061.874762
CGAATAA2100.058.69025145
TTACGAC1251.8189894E-1257.9997752
GCGAAAG26050.055.383663
ATATCGT14950.053.8302231
TATCGTG15250.053.2456932
CGTGTAG15000.053.1664625
AAGCAGG140750.049.7582447
AAAGCAG140950.049.3275876
TGACGCC2400.048.333145145
Produced by FastQC (version 0.11.5)