FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM551959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM551959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811760
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA339161.206219591999317No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT217000.7717586138219478No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA194290.6909906962187384No Hit
GTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC183420.6523316357014823No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT166800.5932227501635986No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG162930.5794591288018892No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT150340.5346829032349845No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT145490.5174339203914986No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTC134870.47966398270122623No Hit
GTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAAC128710.45775599624434515No Hit
AGCGAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC128410.45668904885196465No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT121640.432611602697243No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT110250.39210316669986056No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC109360.3889378894357982No Hit
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGA109090.38797763678265573No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAG107910.3837809770392921No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT100200.3563604290551114No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTT99210.3528395026602555No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCT98780.35131021139784335No Hit
AGCGAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTC96480.3431302813895923No Hit
AAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGT95900.3410675164309898No Hit
ACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGG95420.33936040060318096No Hit
CCCATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCG89130.31699007027626824No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA88460.31460722109995165No Hit
GTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCAG87410.31087290522661964No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGC79560.282954448459328No Hit
AGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAG79490.28270549406777246No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCC73560.2616155006117165No Hit
AAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTG71560.254502517995846No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCA70690.25140837055794235No Hit
GATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGTGA69280.24639371781375366No Hit
GGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCT68970.24529120550829375No Hit
ATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAAC62190.22117819444049278No Hit
ATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCTCTCGC61050.21712379434944662No Hit
AGCGAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATC59660.21218027143141663No Hit
GAGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTT57730.2053162432071016No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA57450.20432042564087974No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT57430.20424929581472104No Hit
CCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA57160.2032890431615785No Hit
GGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCTAACC56310.20026602554983355No Hit
GGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACA54560.19404216576094688No Hit
GTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCAGACCCACCATT54260.19297521836856632No Hit
GGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGT52760.18764048140666342No Hit
CCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGC52020.18500867783879138No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA51530.1832659970979031No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA51030.18148775144393547No Hit
GGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAA50700.18031410931231684No Hit
AAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTA50140.1783224741798731No Hit
CTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGG50110.17821577944063505No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT48720.17327225652260506No Hit
GAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCTCTCGCTATTGCCGC48170.17131618630324066No Hit
GTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTC47930.17046262838933623No Hit
ATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGTTCCTT47640.169431245910035No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG47590.16925342134463825No Hit
AAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGT47290.16818647395225766No Hit
CGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTC47160.16772413008222609No Hit
GTTCAAGTGATCCTCTCGCTATTGCCGCAAATATCATTGGGATCTTGCAC47140.16765300025606739No Hit
GTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTC46740.16623040373289327No Hit
ATGTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCT46610.1657680598628617No Hit
GTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC46150.16413207386121148No Hit
GAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAG46130.16406094403505278No Hit
CACGTATCTCGATCCGCAAGCGCTGAACGGCACCAACGACCTGCGGCTCG46060.1638119896434973No Hit
GAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCT45940.16338521068654507No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTC45290.16107349133638718No Hit
CCACTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGC44740.1591174211170228No Hit
CTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGG44370.15780151933308675No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG44190.1571613508976584No Hit
GTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCT42910.15260904202350128No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA42150.14990610862947049No Hit
GGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACC40440.14382450849290124No Hit
CATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCA39730.14129939966426722No Hit
CAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCCTGTC39680.14112157509887047No Hit
GTCTCCGGTTACTCGGGAGGCGGAAGGGCCGCCGTCGAAGCATTCGAATC38950.13852533644407775No Hit
GTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCA38770.1378851680086494No Hit
GACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAA38750.1378140381824907No Hit
TTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAAT38630.13738725922553843No Hit
GAGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA38300.13621361709391983No Hit
CAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCC38120.1355734486584915No Hit
GTTGAGCGCTCACGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTC37910.13482658548382506No Hit
ATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACG36990.13155461348052466No Hit
AGCGAAAGCAGGCCGACGTACGCGCGGCGGCCGAACATCGTGTTCCGGTA36970.13148348365436593No Hit
AGATACGTGATTGCGTGCGCGTCAGCCAGCGGCGTGACGTCGACGTGACG36590.13013201695735055No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT36360.12931402395652544No Hit
ATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGC36000.12803368708566876No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCCAAG35810.12735795373716108No Hit
GCGTTCGACAGTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGA35320.12561527299627281No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAG35150.1250106694739238No Hit
AGACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCA35100.12483284490852704No Hit
ACCCCCGCCTTATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCT35000.12447719577773353No Hit
CCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTG34480.12262782029760719No Hit
TCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCG34270.1218809571229408No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA34240.12177426238370273No Hit
GGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCGAACCAA34210.12166756764446468No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC34170.12152530799214728No Hit
GCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATC33920.12063618516516346No Hit
GCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGC33690.11981819216433835No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCT33650.11967593251202094No Hit
AGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCA33350.11860898511964037No Hit
AAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGT33220.11814664124960877No Hit
AGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTC31990.11377215694084843No Hit
CTTTAAATACGGACTGAAAGGAGGGCCTTCTACGGAAGGAGTGCCAAAGT31790.11306085867926138No Hit
GGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATT31730.11284746920078527No Hit
TTCTAGAGCTTCTCGCCAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT31430.11178052180840471No Hit
AAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGG30900.10989558141519902No Hit
TTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGCGGATCGGCCTGCCGG30690.10914871824053263No Hit
ATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTT29820.10605457080262895No Hit
CTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGA29780.10591231115031155No Hit
GTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGG29750.10580561641107349No Hit
GCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAA29740.10577005149799415No Hit
GAGCGAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTT29330.10431189006174069No Hit
TTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAG29060.10335163740859818No Hit
GCACTTGACATTGTGGATTCTTGATCGTCTTTTTTTCAAATGCATTTACC28840.10256920932085242No Hit
TGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGG28800.10242694966853501No Hit
TTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGC28590.1016800864938686No Hit
GTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTT28540.10150226192847185No Hit
TATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCA28420.10107548297151962No Hit
GATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATC28400.10100435314536092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA14700.0135.629611
AGCGAAA80600.0118.285051
GAGCAAA25000.0117.16031
GCGAAAG90300.0105.657312
CGAAAGC93150.0102.34683
GAAAGCA101900.093.771894
AGTAGAA61050.091.797941
AGCAAAA34600.084.232822
TATTAGT4050.073.395251
TAGAAAC84350.066.095623
AGAAACA88200.063.128294
ACGGAGT350.003567390862.141914145
GGCATAA350.003567390862.141914145
GCAAAAG47200.061.900543
GTAGAAA92550.060.7095032
CGATTCC851.3065801E-859.704975145
CCGGGCC3200.056.639763145
ACAAGGT168950.053.9396933
AAAGCAG209050.053.754265
ACTAATA6650.052.3300328
Produced by FastQC (version 0.11.5)