FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM551457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM551457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786150
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA234980.843386034492041No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT201150.7219640005024854No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA194670.6987060998151571No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT178750.6415663191141898No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT165190.5928970084166323No Hit
GTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC154690.5552105952658687No Hit
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGA152160.546129964287637No Hit
GTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAAC131830.473161890063349No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG127020.4558979236580945No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTC105010.37690002332968436No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT102960.3695421998097733No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT98080.3520269906501804No Hit
ACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGG93730.33641404805914976No Hit
GTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCT90990.32657968881790284No Hit
TCATACAATGGTCGGAACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAAT87420.3137663083466432No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT87280.3132638228379664No Hit
CCCATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCG87080.3125459863969995No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGC83500.2996967141036915No Hit
GTTAAGACCGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTGGTG82730.2969330438059688No Hit
AGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAG82390.295712721856325No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTT76950.27618757066202465No Hit
GAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAG76890.27597221972973457No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAG75730.2718087683721264No Hit
GTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCAG74690.26807601887909843No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCT74030.26570715862390754No Hit
CTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA73300.26308705561437823No Hit
ATGTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCT73120.26244100281750804No Hit
CCTTAGAGTGCTCTTGCGTAGCAACTAAGGACAAGGGTTGCGCTCGTTGC70700.25375518188180823No Hit
CCCTAAACGATGTCAACTAGTTGTTGGGGATTCATTTCCTTAGTAACGTA69760.25038135060926364No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA69140.2481560576422662No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCC67210.24122893598693537No Hit
TGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTGGCT67150.2410135850546453No Hit
TTCTAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT67010.24051109954596844No Hit
GGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCTAACC65890.23649121547655366No Hit
AGCGAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC65670.23570159539149008No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCAAAG64590.23182527861026866No Hit
ACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTT62050.2227087558099887No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTC61740.22159610932648996No Hit
GGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTT61480.22066292195323298No Hit
ATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAAC59290.2128026129246451No Hit
TGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCA59010.21179764190729142No Hit
CCCTTGACATGGTCGGAATTCCGCTGAGAGGTGGAAGTGCTCGAAAGAGA58260.20910575525366543No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA58240.20903397160956877No Hit
CTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGG54900.19704610304542108No Hit
ATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGT53910.19349281266263482No Hit
GGCCAATACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAG53230.19105216876334727No Hit
TTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTC52100.18699639287188416No Hit
GCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGC51910.18631444825296556No Hit
TCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCG51770.1858119627442887No Hit
GAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCT51330.18423272257416148No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAG51030.18315596791271108No Hit
CCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGC50750.18215099689535738No Hit
GTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCAGACCCACCATT50530.18136137681029377No Hit
CGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTC49840.17888484108895789No Hit
CCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA49700.17838235558028104No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGC49500.1776645191393141No Hit
CTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGA48720.1748649570195431No Hit
GGATATAGGCCGCCACGAACAGGCAGGCAACCCCAAGCAGGATGAACCCG47270.1696606428225329No Hit
GTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTC46610.16729178256734203No Hit
GTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGG46120.16553308328697305No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCT44960.1613696319293649No Hit
CCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTTGGGGATTCAT44870.16104660553092978No Hit
TCCTTAGAGTGCTCTTGCGTAGCAACTAAGGACAAGGGTTGCGCTCGTTG43960.15778044972453026No Hit
GAGTATGGCAGAGGGGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG43550.15630888502054807No Hit
GGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAA43550.15630888502054807No Hit
GTCATGAATCCTACCGTGGTGACCGTCCTCCTTGCGGTTAGACTAGCCAC43470.1560217504441613No Hit
ACCCCCGCCTTATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCT43260.15526802218114602No Hit
CTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGG42140.15124813811173124No Hit
GTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTA41990.15070976078100606No Hit
GATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGTGA41890.15035084256052259No Hit
ATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACG41730.14977657340774905No Hit
GTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT40520.14543366293989912No Hit
GGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACG40510.1453977711178508No Hit
AGACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCA40480.14529009565170575No Hit
ATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTT40390.14496706925327063No Hit
GAGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA40240.14442869192254545No Hit
GGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACA40190.1442492328123037No Hit
GTTGAGCGCTCACGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTC40020.1436390718374818No Hit
GTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCA39190.14066005060746908No Hit
TCCCTATCCGATCCTGAACGTGCTTTTCAGCACGTCGCGCAGATGCAGCG39160.14055237514132404No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGGTGTACCG38990.13994221416650218No Hit
GGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACC38620.13861421675071336No Hit
CCTTTCTAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA38260.1373221111569729No Hit
GTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTC38250.13728621933492452No Hit
ACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACG37840.13581465463094233No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCAAAGTT37490.13455844085925023No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGAT37070.13305098433321969No Hit
AGCGAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATC36790.13204601331586596No Hit
CTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTGG36740.13186655420562424No Hit
GGTCGGAACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCAGAAAA36220.13000017945911024No Hit
ATACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACC35910.1288875329756115No Hit
ACCCTTGACATGGTCGGAATTCCGCTGAGAGGTGGAAGTGCTCGAAAGAG35230.12644688907632395No Hit
CCTTAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTC35010.12565726899126034No Hit
GTATGGCAGAGGGGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAGAG34890.1252265671266802No Hit
CTTATGGGTAGGGCTTCACACGTCATACAATGGTCGGAACAGAGGGTTGC34560.12404213699908477No Hit
GCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATC34140.12253468047305421No Hit
TGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGG34120.12246289682895752No Hit
ATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGC33710.12099133212497533No Hit
AAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGT33670.12084776483678195No Hit
CAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCC33530.1203452793281051No Hit
GGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGT33040.11858658004773612No Hit
CAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCCTGTC32980.11837122911544605No Hit
AAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTA32910.11811998636110763No Hit
GACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAA31930.11460258780036968No Hit
GGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATT31390.11266442940975899No Hit
GCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCCCCTGGGCCAA31370.11259264576566229No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGGTGTACCGC31360.11255675394361395No Hit
CCCATGTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGG31320.11241318665542055No Hit
GGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCGAACCAA31050.11144410746011521No Hit
CCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTG30970.11115697288372843No Hit
CACCATGGGAGTGGGTTTTACCAGAAGTGGCTAGTCTAACCGCAAGGAGG30950.11108518923963176No Hit
GTCCTTAGTTGCTACGCAAGAGCACTCTAAGGAGACTGCCGGTGACAAAC30710.11022378551047143No Hit
CATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCA30580.10975719182384294No Hit
ACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTC30500.10947005724745618No Hit
GTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCT30490.10943416542540782No Hit
GGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGA30300.10875222080648925No Hit
CTGCAACTCGAGTGCATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCA30040.10781903343323224No Hit
CTTCCACCTCTCAGCGGAATTCCGACCATGTCAAGGGTAGGTAAGGTTTT29960.10753189885684547No Hit
TTCATTTCCTTAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAG29890.10728065610250705No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACC29770.1068499542379269No Hit
TTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAAT29590.10620390144105665No Hit
TTCCGATACGGCTACCTTGTTACGACTTCACCCCAGTCATGAATCCTACC29030.10419395940634926No Hit
ATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCTCTGGAAA28840.10351201478743068No Hit
GTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCAT28810.10340433932128565No Hit
GTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACA28610.1026865028803187No Hit
CCACTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGC28350.10175331550706171No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGG28210.10125082999838486No Hit
GTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCA27910.10017407533693448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11450.0129.801351
AGTAGAA27650.096.4902651
GAAAGCA74950.081.639734
GCAAAAG26250.080.093923
CAAAAGC26850.078.304114
CCGGGCC1550.074.83747145
AGCGAAA83200.073.1087651
GGATATA10650.070.796941
GCGAAAG90800.067.46862
AAAGCAG105350.066.47735
GTTAATG451.1943293E-464.4433753
GATATAG11700.064.4433752
TAGAAAC44300.060.2247353
GTAGAAA44400.059.9258082
AAACAAG108350.058.2131541
CGATAAA1400.056.96334145
CTACTCC5700.055.96398145
AGAAACA48000.055.733454
ATATAGG13400.055.726693
CTAATAA5250.055.237189
Produced by FastQC (version 0.11.5)