FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM551087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM551087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020545
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA121480.3021480918631678No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA77420.1925609587754894No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC75210.187064191546171No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT75040.18664136329776188No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC72500.18032381182153168No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG64970.16159500764199877No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT52510.13060418425860176No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT49370.12279429778798646No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC48870.12155068529266554No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC48810.12140145179322705No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT47520.1181929315552991No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA43080.10714965259684944No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT42950.106826313348066No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC41520.1032695816114482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA25450.092.586741
GAGCAAA124400.076.174291
GCAAAAG128500.072.386093
AGCAAAA133950.069.928052
CAAAAGC135050.069.4121554
CGAAAGC36050.065.761864
AGCGAAA36350.064.022442
CGAATAA2000.057.99987145
GCGAAAG41600.056.8140683
TATCGTG28850.054.531262
CGTGTAG28800.054.37425
ATATCGT28750.054.219291
AAAGCAG216350.053.8506056
AAGCAGG217250.053.5274057
GCGCTTC901.3247172E-648.332626
ATAAGTG60050.048.0509158
AAAAGCA199050.047.822715
ATCGTGT33250.047.315093
AGCAGGG84050.047.1825268
TCGTGTA34600.045.4689834
Produced by FastQC (version 0.11.5)