FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM550443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM550443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3185242
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG117100.3676329773373577No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG115450.3624528371784624No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT113920.3576494344856686No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC112750.3539762441911792No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC106120.33316149918907256No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT101700.31928500252100156No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA100430.31529786433809426No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGGT76700.2407980304165272No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC73540.23087727714252168No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGGTGTGTG66590.2090578988974778No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG64900.20375217958321531No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG64700.20312428380637954No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA63720.20004759449988416No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG61550.1932349253212158No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT59260.18604551867644595No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA56860.1785107693544164No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGAACAAAGCGTCTACG56610.17772589963337165No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT55250.17345620835088826No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT52840.16589006424001693No Hit
TTCCCATTGAGGGCATTTTGAACAAAGCGTCTACGCTGCAGTCCTCGCTC51150.16058434492575446No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA49230.15455654546813083No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACCAGAAACGAAT48840.15333214870330103No Hit
CACCAGGCCAAATGCTACTTCAGTGGTTACAGCCCCCATCCTATTGTATA48550.15242169982688913No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC48200.1513228822174265No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGGTGT47090.14783806065598784No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC46500.14598576811432226No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA46470.14589158374779687No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG45370.14243815697520001No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT45340.14234397260867462No Hit
GACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAGTGAGC42320.13286274637845413No Hit
CTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCATGGCCTC41190.12931513523933189No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACT40920.12846747594060356No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC40410.1268663417096723No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC40350.12667797297662156No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC40330.12661518339893799No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA39640.12444894296885448No Hit
ATATTGTGGATTCTTGATCGTCTTTTTTTCAAATGCGTCTATCGACTCTT39430.1237896524031769No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT39390.12366407324780974No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGAAC39130.12284780873792323No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG38850.12196875465035309No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC37290.1170711675910339No Hit
TCTATCGACTCTTCAAACACGGCCTTAAAAGAGGCCCTTCTACGGAAGGA37200.11678861449145779No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT37060.11634908744767275No Hit
GACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATT36680.11515608547168472No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA36630.11499911152747577No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG36530.11468516363905788No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGG36480.11452818969484894No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG36450.11443400532832357No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC35270.11072942024499238No Hit
CTTCAAACACGGCCTTAAAAGAGGCCCTTCTACGGAAGGAGTACCTGAGT34430.10809225798228204No Hit
CCTCTGACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAG34340.10780970488270593No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG34170.10727599347239551No Hit
GGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCC33840.10623996544061644No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC33110.10394814585516579No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT33010.1036341979667479No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA32590.10231561683539273No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA32430.10181330021392408No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTAGGG32370.10162493148087334No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG32320.10146795753666439No Hit
GTTCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC32070.10068308781561966No Hit
GTCTTTAGCCATTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAA31890.10011798161646746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17700.088.885891
CGAAAGC18350.085.733313
CTCGACA353.438908E-582.8548364
GCGAAAG20350.077.3074342
GCATACG502.0111904E-457.998392
TAGAAAC25850.054.128092
TTACGCC3550.053.09795145
AGTAGAA25000.046.4008941
ATACGTG700.00105713441.4274184
ACGATCC4600.040.97776145
TGACGCC5900.039.321556145
CAAGGTA23700.037.6255344
AAGGTAG24900.036.1034135
GAGCGAA8300.035.8139421
ACTATTC3650.035.7524268
GGTAGTT22650.035.2087337
GGGTTAA4750.035.105941
GTAGAAA43700.034.8404921
AAACGCG1251.2661951E-534.7990345
GGCATAC1051.8544133E-434.5244751
Produced by FastQC (version 0.11.5)