FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM550272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM550272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2004894
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA38670.19287802746678878No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC31090.15507054238278933No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA26100.130181446001634No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC25900.1291838870284414No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC21700.10823514859139687No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC21020.10484344808254203No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT21010.1047935701338824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11550.095.408451
GAGCAAA56300.068.505981
GCAAAAG55700.068.462983
CAAAAGC58250.066.3371054
AGCAAAA59950.063.488542
CGAAAGC17350.062.678274
AGCGAAA17400.062.4981652
GCGAAAG19050.057.0849343
AAAAGCA79050.049.432455
AAAGCAG100050.049.201456
AAGCAGG103250.047.6765637
ATATCGT11950.046.107431
TATCGTG12750.044.920252
TCGTGTA14150.039.4511384
TCGTACT2950.039.3208772
ATCGTGT14650.038.1046833
AGCAGGG39200.037.5444768
TATAAGT31900.037.2716337
ATATAAG32550.036.97286
AGCAGGA37500.036.3465048